Regulated Apoptosis of Genetically Modified Hematopoietic Stem and Progenitor Cells Via an Inducible Caspase‐9 Suicide Gene in Rhesus Macaques

• Published in: Stem cells (Dayton, Ohio) Vol. 33; no. 1; pp. 91 - 100
• Main Authors: Barese, Cecilia N., Felizardo, Tania C., Sellers, Stephanie E., Keyvanfar, Keyvan, Di Stasi, Antonio, Metzger, Mark E., Krouse, Allen E., Donahue, Robert E., Spencer, David M., Dunbar, Cynthia E.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2015
• Subjects: Animals; Apoptosis - physiology; Caspase 9 - biosynthesis; Caspase 9 - genetics; Gene therapy; Genes; Genes, Transgenic, Suicide; Genetic Therapy; Genetic Vectors; Genotoxicity; Hematopoietic Stem Cell Transplantation - methods; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - enzymology; HSC transplantation; iCasp9; Macaca mulatta; Medical research; Primates; Risk reduction; Safety; Stem cells; Suicide; Suicide gene; HSC transplantation; Gene therapy; Suicide gene; Genotoxicity; iCasp9

The high risk of insertional oncogenesis reported in clinical trials using integrating retroviral vectors to genetically modify hematopoietic stem and progenitor cells (HSPCs) requires the development of safety strategies to minimize risks associated with novel cell and gene therapies. The ability t...