Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato

• Published in: Plant biotechnology journal Vol. 18; no. 10; pp. 2133 - 2143
• Main Authors: Vu, Tien Van, Sivankalyani, Velu, Kim, Eun‐Jung, Doan, Duong Thi Hai, Tran, Mil Thi, Kim, Jihae, Sung, Yeon Woo, Park, Minwoo, Kang, Yang Jae, Kim, Jae‐Yean
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.10.2020; John Wiley and Sons Inc
• Subjects: Alleles; Antibiotics; Binding sites; biotechnology; Cell culture; Cloning; CRISPR; CRISPR/Cas9; CRISPR/Cpf1; Deoxyribonucleic acid; DNA; Editing; Efficiency; gene targeting; Genetic transformation; genome editing; Genomes; genomics; Germination; Heredity; Homology; homology‐directed repair; multi‐replicon; Mutation; Non-homologous end joining; Offspring; phenotypic selection; Plant cells; progeny; Proteins; Repair; replicon; Reproduction (biology); salt tolerance; self-pollination; Sodium chloride; temperature; Tomatoes; CRISPR/Cpf1; gene targeting; genome editing; multi-replicon; CRISPR/Cas9; homology-directed repair

Summary Genome editing via the homology‐directed repair (HDR) pathway in somatic plant cells is very inefficient compared with error‐prone repair by nonhomologous end joining (NHEJ). Here, we increased HDR‐based genome editing efficiency approximately threefold compared with a Cas9‐based single‐repl...