Real‐time RT‐PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root‐ and shoot‐specific genes

• Published in: The Plant journal : for cell and molecular biology Vol. 38; no. 2; pp. 366 - 379
• Main Authors: Czechowski, Tomasz, Bari, Rajendra P., Stitt, Mark, Scheible, Wolf‐Rüdiger, Udvardi, Michael K.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science, Ltd 01.04.2004; Blackwell Science
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Base Sequence; Biological and medical sciences; DNA primers; DNA Primers - genetics; DNA, Complementary; DNA, Complementary - genetics; DNA, Plant; DNA, Plant - genetics; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Profiling; Genes, Plant; genetics; metabolism; methods; Molecular and cellular biology; Molecular genetics; Oligonucleotide Array Sequence Analysis; Plant Roots; Plant Roots - metabolism; Plant Shoots; Plant Shoots - metabolism; rapid methods; real‐time RT‐PCR; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - statistics & numerical data; root; roots; Sensitivity and Specificity; shoot; shoots; statistics & numerical data; transcript profiling; transcription (genetics); transcription factor; transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription. Transcription factor. Splicing. Rna processing; Arabidopsis; transcript profiling; real-time RT-PCR; Arabidopsis thaliana; Polymerase chain reaction; Investigation method; Cruciferae; Dicotyledones; Angiospermae; root; Spermatophyta; shoot; Transcription factor; Expressed sequence tag

Summary To overcome the detection limits inherent to DNA array‐based methods of transcriptome analysis, we developed a real‐time reverse transcription (RT)‐PCR‐based resource for quantitative measurement of transcripts for 1465 Arabidopsis transcription factors (TFs). Using closely spaced gene‐speci...