Using clear plastic CD cases as low‐cost mini‐rhizotrons to phenotype root traits

Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizo...

Full description

Saved in:
Bibliographic Details
Published inApplications in plant sciences Vol. 8; no. 4; pp. e11340 - n/a
Main Authors Cassidy, Steven T., Burr, Audrey A., Reeb, Rachel A., Melero Pardo, Ana L., Woods, Kamron D., Wood, Corlett W.
Format Journal Article
LanguageEnglish
Published United States John Wiley & Sons, Inc 01.04.2020
John Wiley and Sons Inc
Wiley
Subjects
Farms
Laboratories
Legumes
Medicago
Methods
mini‐rhizotron
Phenotypes
Plant resistance
Plant sciences
Protocol Note
Protocol Notes
Researchers
rhizobia
Rhizosphere
root phenotype
Seed germination
Seeds
symbiosis
United States > US
mini‐rhizotron
Medicago
root phenotype
rhizobia
symbiosis
Online AccessGet full text

Cover

Abstract Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizotron alternatives. Methods and Results The method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc (CD) cases, we developed mini‐rhizotrons for use in the field and laboratory using the burclover Medicago lupulina. Conclusions Our method combines the benefits of pots and germination pouches. In CD mini‐rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.
AbstractList PremiseWe developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizotron alternatives.Methods and ResultsThe method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc (CD) cases, we developed mini‐rhizotrons for use in the field and laboratory using the burclover Medicago lupulina.ConclusionsOur method combines the benefits of pots and germination pouches. In CD mini‐rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.
Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizotron alternatives. Methods and Results The method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc ( CD ) cases, we developed mini‐rhizotrons for use in the field and laboratory using the burclover Medicago lupulina . Conclusions Our method combines the benefits of pots and germination pouches. In CD mini‐rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.
We developed a novel low-cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long-term experiments and the high cost of other mini-rhizotron alternatives. The method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc (CD) cases, we developed mini-rhizotrons for use in the field and laboratory using the burclover . Our method combines the benefits of pots and germination pouches. In CD mini-rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.
Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizotron alternatives. Methods and Results The method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc (CD) cases, we developed mini‐rhizotrons for use in the field and laboratory using the burclover Medicago lupulina. Conclusions Our method combines the benefits of pots and germination pouches. In CD mini‐rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.
Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address the difficulties encountered growing plants in seed germination pouches for long‐term experiments and the high cost of other mini‐rhizotron alternatives. Methods and Results The method described here took inspiration from homemade ant farms commonly used as an educational tool in elementary schools. Using compact disc (CD) cases, we developed mini‐rhizotrons for use in the field and laboratory using the burclover Medicago lupulina. Conclusions Our method combines the benefits of pots and germination pouches. In CD mini‐rhizotrons, plants grew significantly larger than in germination pouches, and unlike pots, it is possible to measure roots without destructive sampling. Our protocol is a cheaper, widely available alternative to more destructive methods, which could facilitate the study of belowground phenotypes and processes by scientists with fewer resources.
Author Burr, Audrey A.
Melero Pardo, Ana L.
Reeb, Rachel A.
Cassidy, Steven T.
Woods, Kamron D.
Wood, Corlett W.
AuthorAffiliation 1 Department of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USA
2 Present address: Department of Biology University of Florida Gainesville Florida USA
AuthorAffiliation_xml – name: 1 Department of Biological Sciences University of Pittsburgh Pittsburgh Pennsylvania USA
– name: 2 Present address: Department of Biology University of Florida Gainesville Florida USA
Author_xml – sequence: 1
  givenname: Steven T.
  orcidid: 0000-0003-4569-5910
  surname: Cassidy
  fullname: Cassidy, Steven T.
  email: stevencassidy@ufl.edu
  organization: University of Pittsburgh
– sequence: 2
  givenname: Audrey A.
  surname: Burr
  fullname: Burr, Audrey A.
  organization: University of Pittsburgh
– sequence: 3
  givenname: Rachel A.
  surname: Reeb
  fullname: Reeb, Rachel A.
  organization: University of Pittsburgh
– sequence: 4
  givenname: Ana L.
  surname: Melero Pardo
  fullname: Melero Pardo, Ana L.
  organization: University of Pittsburgh
– sequence: 5
  givenname: Kamron D.
  surname: Woods
  fullname: Woods, Kamron D.
  organization: University of Pittsburgh
– sequence: 6
  givenname: Corlett W.
  surname: Wood
  fullname: Wood, Corlett W.
  organization: University of Pittsburgh
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32351801$$D View this record in MEDLINE/PubMed
BookMark eNp9ks9qVDEUh4NUbK3d-AAScCPCtPl_k41QRm0LhQo665Dk5s5kuJNckzuWceUj-Iw-iWlvLa2LZpPDycdHzuH3EuzFFD0ArzE6xgiREzMUeowxZegZOCBYyBliHO09qPfBUSlrVI-sLcVegH1KKMcS4QOwWJQQl9D13mQ49KaMwcH5R-hM8QWaAvt0_efXb5fKCDchhlrnVfiZxpxigWOCw8rHNO4GD3NKIxyzCWN5BZ53pi_-6O4-BIvPn77Nz2eXV2cX89PLmeOMoFlrTItb1HiFO47bFksirXDcYmsdIcSTljBkjOqkbXiLBWssxcIppZBHvKGH4GLytsms9ZDDxuSdTibo20bKS21ynaj3mhnLiFXccaeYQp3xQgqPJLNOSeW66vowuYat3fjW-Vhn6R9JH7_EsNLL9EM3WNa1iip4dyfI6fvWl1FvQnG-7030aVs0oUpILoSiFX37H7pO2xzrqjRhXLKGokY9SVHFcYOFIpV6P1Eup1Ky7-6_jJG-iYi-iYi-jUiF3zwc8h79F4gK4Am4Dr3fPaHSp1--0kn6FyRryLo
CitedBy_id crossref_primary_10_1002_ecy_3720
crossref_primary_10_1042_ETLS20200275
crossref_primary_10_3390_microorganisms10071380
crossref_primary_10_1002_aps3_11341
crossref_primary_10_1093_insilicoplants_diab036
crossref_primary_10_3389_fmicb_2021_625752
crossref_primary_10_3390_plants12244150
Cites_doi 10.1002/evl3.51
10.4141/cjps79-015
10.1002/ece3.3012
10.1007/BF02139633
10.1186/s13007-015-0046-x
10.1163/15685411-00003184
10.1007/s00374-016-1173-x
10.1007/s10681-012-0686-0
10.2134/agronj1988.00021962008000020029x
10.1186/1746-4811-9-3
10.1007/s11104-005-4887-3
10.1186/s13007-016-0131-9
10.2307/2445879
10.1080/17550874.2010.517788
10.1105/tpc.114.124032
10.1007/BF02257535
10.1038/nmeth.2019
10.1186/s12870-019-1791-1
10.1093/jxb/erp083
10.1186/1746-4811-9-38
10.1080/03650340.2017.1342034
10.1007/s11104-010-0623-8
10.1093/aob/mcu009
10.1016/j.soilbio.2012.05.020
10.1104/pp.111.179895
10.1007/978-3-642-22067-8_17
ContentType Journal Article
Copyright 2020 Cassidy et al. is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America
2020 Cassidy et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.
2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
Copyright_xml – notice: 2020 Cassidy et al. is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America
– notice: 2020 Cassidy et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.
– notice: 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
DBID 24P
WIN
NPM
AAYXX
CITATION
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
GNUQQ
HCIFZ
LK8
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.1002/aps3.11340
DatabaseName Wiley Open Access
Wiley Free Archive
PubMed
CrossRef
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
AUTh Library subscriptions: ProQuest Central
ProQuest Natural Science Collection
ProQuest One Community College
ProQuest Central
ProQuest Central Student
SciTech Premium Collection (Proquest) (PQ_SDU_P3)
Biological Sciences
Biological Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle PubMed
CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest Biological Science Collection
ProQuest Central Essentials
ProQuest One Academic Eastern Edition
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
Biological Science Database
ProQuest SciTech Collection
ProQuest Central China
ProQuest Central
ProQuest One Academic UKI Edition
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest One Academic
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database
CrossRef
PubMed

MEDLINE - Academic
Publicly Available Content Database
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: 24P
  name: Wiley Open Access
  url: https://authorservices.wiley.com/open-science/open-access/browse-journals.html
  sourceTypes: Publisher
– sequence: 3
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Botany
DocumentTitleAlternate Mini‐rhizotron alternative in Medicago
EISSN 2168-0450
EndPage n/a
ExternalDocumentID oai_doaj_org_article_4ab42b95c5c9490fae686e084bc989cf
10_1002_aps3_11340
32351801
APS311340
Genre article
Journal Article
GeographicLocations United States--US
GeographicLocations_xml – name: United States--US
GrantInformation_xml – fundername: University of Pittsburgh Department of Biological Sciences
GroupedDBID 0R~
1OC
24P
5VS
8FE
8FH
AAEFD
AAHBH
AAHHS
AAPSS
ABDBF
ACCFJ
ACGFO
ACGFS
ACXQS
ADBBV
ADEHT
ADHSS
ADKYN
ADRAZ
ADRYA
ADZMN
ADZOD
AEEZP
AEGXH
AENEX
AEPYG
AEQDE
AFKRA
AFNWH
AIAGR
AIWBW
AJBDE
AKPMI
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AOIJS
AVUZU
BBNVY
BCNDV
BENPR
BHPHI
CCPQU
EBS
EJD
GROUPED_DOAJ
GX1
H13
HCIFZ
HYE
IAO
IGS
IPNFZ
KQ8
LK8
M48
M7P
M~E
O9-
OK1
PIMPY
PQ0
PROAC
RBO
RIG
RNS
ROL
RPM
RZN
TBO
WIN
ITC
NPM
AAYXX
CITATION
ABUWG
AZQEC
DWQXO
GNUQQ
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
ID FETCH-LOGICAL-c5420-daad1d07e91f51dd1828b6c5b1bbc222e2d240aa9f8b75d1647b316c9990e0573
IEDL.DBID RPM
ISSN 2168-0450
IngestDate Tue Oct 22 15:16:09 EDT 2024
Tue Sep 17 21:22:49 EDT 2024
Fri Jun 28 20:16:52 EDT 2024
Thu Oct 10 17:40:03 EDT 2024
Thu Oct 10 17:18:47 EDT 2024
Thu Sep 26 16:08:39 EDT 2024
Sat Nov 02 12:23:13 EDT 2024
Sat Aug 24 01:06:54 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords mini‐rhizotron
Medicago
root phenotype
rhizobia
symbiosis
Language English
License Attribution
2020 Cassidy et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c5420-daad1d07e91f51dd1828b6c5b1bbc222e2d240aa9f8b75d1647b316c9990e0573
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0003-4569-5910
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186896/
PMID 32351801
PQID 2395171692
PQPubID 4368373
PageCount 7
ParticipantIDs doaj_primary_oai_doaj_org_article_4ab42b95c5c9490fae686e084bc989cf
pubmedcentral_primary_oai_pubmedcentral_nih_gov_7186896
proquest_miscellaneous_2396856693
proquest_journals_2458473079
proquest_journals_2395171692
crossref_primary_10_1002_aps3_11340
pubmed_primary_32351801
wiley_primary_10_1002_aps3_11340_APS311340
PublicationCentury 2000
PublicationDate April 2020
PublicationDateYYYYMMDD 2020-04-01
PublicationDate_xml – month: 04
  year: 2020
  text: April 2020
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
– name: Hoboken
PublicationTitle Applications in plant sciences
PublicationTitleAlternate Appl Plant Sci
PublicationYear 2020
Publisher John Wiley & Sons, Inc
John Wiley and Sons Inc
Wiley
Publisher_xml – name: John Wiley & Sons, Inc
– name: John Wiley and Sons Inc
– name: Wiley
References 2012; 61
2017; 7
2012; 186
2011; 157
1997; 84
1979; 59
2004; 267
2012
2009; 60
2015; 11
2014; 26
2019; 19
2006
1985; 85
1996; 185
2018; 64
2012; 55
2018; 20
2014; 114
2013; 9
2016; 12
2017; 53
2018; 2
2019
2018
2016
2010; 3
1988; 80
2011; 341
2012; 9
Barker D. G. (e_1_2_6_3_1) 2006
e_1_2_6_10_1
e_1_2_6_31_1
e_1_2_6_30_1
R Core Team (e_1_2_6_22_1) 2018
e_1_2_6_19_1
e_1_2_6_13_1
e_1_2_6_14_1
e_1_2_6_11_1
e_1_2_6_12_1
e_1_2_6_33_1
e_1_2_6_17_1
e_1_2_6_18_1
e_1_2_6_15_1
e_1_2_6_16_1
Atamian H. S. (e_1_2_6_2_1) 2012; 61
e_1_2_6_21_1
e_1_2_6_20_1
Yates R. J. (e_1_2_6_32_1) 2016
e_1_2_6_9_1
Garcia J. (e_1_2_6_8_1) 2006
e_1_2_6_5_1
e_1_2_6_4_1
e_1_2_6_7_1
e_1_2_6_6_1
e_1_2_6_25_1
e_1_2_6_24_1
e_1_2_6_23_1
e_1_2_6_29_1
e_1_2_6_28_1
e_1_2_6_27_1
e_1_2_6_26_1
References_xml – volume: 85
  start-page: 291
  year: 1985
  end-page: 293
  article-title: A root observation and sampling chamber (rhizotron) for pot studies
  publication-title: Plant and Soil
– volume: 3
  start-page: 151
  year: 2010
  end-page: 164
  article-title: Biomass and productivity of fine and coarse roots in five tropical mountain forests stands along an altitudinal transect in southern Ecuador
  publication-title: Plant Ecology & Diversity
– volume: 26
  start-page: 2390
  year: 2014
  end-page: 2403
  article-title: A scalable open‐source pipeline for large‐scale root phenotyping of
  publication-title: Plant Cell
– volume: 19
  start-page: 180
  year: 2019
  article-title: Size matters: Three methods for estimating nuclear size in mycorrhizal roots of by image analysis
  publication-title: BMC Plant Biology
– volume: 341
  start-page: 75
  year: 2011
  end-page: 87
  article-title: Shovelomics: High throughput phenotyping of maize ( L.) root architecture in the field
  publication-title: Plant and Soil
– volume: 59
  start-page: 99
  year: 1979
  end-page: 110
  article-title: The biology of Canadian weeds. 33. L
  publication-title: Canadian Journal of Plant Sciences
– volume: 2
  start-page: 233
  year: 2018
  end-page: 245
  article-title: Genetic conflict with a parasitic nematode disrupts the legume–rhizobia mutualism
  publication-title: Evolution Letters
– volume: 53
  start-page: 187
  year: 2017
  end-page: 198
  article-title: Introduced earthworm species exhibited unique patterns of seasonal activity and vertical distribution, and burrows remained usable for at least 7 years in hardwood and pine stands
  publication-title: Biology and Fertility of Soils
– volume: 55
  start-page: 1
  year: 2012
  end-page: 6
  article-title: Soil inoculation method determines the strength of plant–soil interactions
  publication-title: Soil Biology and Biochemistry
– volume: 11
  start-page: 3
  year: 2015
  article-title: “Rhizoponics”: A novel hydroponic rhizotron for root system analyses on mature plants
  publication-title: Plant Methods
– start-page: 73
  year: 2016
  end-page: 108
– volume: 185
  start-page: 293
  year: 1996
  end-page: 304
  article-title: Applications of minirhizotrons to understand root function in forests and other natural ecosystems
  publication-title: Plant and Soil
– year: 2018
– volume: 84
  start-page: 26
  year: 1997
  end-page: 33
  article-title: Fractal geometry of bean root systems: Correlations between spatial and fractal dimension
  publication-title: American Journal of Botany
– volume: 157
  start-page: 29
  year: 2011
  end-page: 39
  article-title: A novel image‐analysis toolbox enabling quantitative analysis of root system architecture
  publication-title: Plant Physiology
– volume: 114
  start-page: 841
  year: 2014
  end-page: 851
  article-title: An integrated method for quantifying root architecture of field‐grown maize
  publication-title: Annals of Botany
– volume: 61
  year: 2012
  article-title: High and low throughput screens with root‐knot nematodes spp
  publication-title: JoVE (Journal of Visualized Experiments)
– volume: 186
  start-page: 897
  year: 2012
  end-page: 905
  article-title: Resistance against broomrapes ( and spp.) in faba bean ( ) based in low induction of broomrape seed germination
  publication-title: Euphytica
– volume: 12
  start-page: 31
  year: 2016
  article-title: RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: Test, comparison with pot grown plants and validation
  publication-title: Plant Methods
– volume: 9
  start-page: 676
  year: 2012
  end-page: 682
  article-title: Fiji: An open‐source platform for biological‐image analysis
  publication-title: Nature Methods
– volume: 64
  start-page: 244
  year: 2018
  end-page: 256
  article-title: Lentil ( L.) growth promoting rhizobacteria and their effect on nodulation in coinoculation with rhizobia
  publication-title: Archives of Agronomy and Soil Science
– volume: 60
  start-page: 1953
  year: 2009
  end-page: 1968
  article-title: Shoot yield drives phosphorus use efficiency in and correlates with root architecture traits
  publication-title: Journal of Experimental Botany
– volume: 9
  start-page: 38
  year: 2013
  article-title: An online database for plant image analysis software tools
  publication-title: Plant Methods
– volume: 80
  start-page: 287
  year: 1988
  end-page: 291
  article-title: Monitoring corn root development with a mini‐rhizotron in confined growth boxes
  publication-title: Agronomy Journal
– volume: 267
  start-page: 225
  year: 2004
  end-page: 234
  article-title: A method to separate plant roots from soil and analyze root surface area
  publication-title: Plant and Soil
– year: 2006
– volume: 20
  start-page: 905
  year: 2018
  end-page: 909
  article-title: Application of seed germination pouch for culture and initial resistance screening of the soybean cyst nematode
  publication-title: Nematology
– volume: 7
  start-page: 4367
  year: 2017
  end-page: 4376
  article-title: No evidence for adaptation to local rhizobial mutualists in the legume
  publication-title: Ecology and Evolution
– year: 2019
– start-page: 341
  year: 2012
  end-page: 361
– volume: 9
  start-page: 3
  year: 2013
  article-title: Rhizo‐lysimetry: Facilities for the simultaneous study of root behaviour and resource use by agricultural crop and pasture systems
  publication-title: Plant Methods
– ident: e_1_2_6_30_1
  doi: 10.1002/evl3.51
– ident: e_1_2_6_27_1
  doi: 10.4141/cjps79-015
– volume: 61
  start-page: e3629
  year: 2012
  ident: e_1_2_6_2_1
  article-title: High and low throughput screens with root‐knot nematodes Meloidogyne spp
  publication-title: JoVE (Journal of Visualized Experiments)
  contributor:
    fullname: Atamian H. S.
– ident: e_1_2_6_10_1
  doi: 10.1002/ece3.3012
– ident: e_1_2_6_12_1
  doi: 10.1007/BF02139633
– ident: e_1_2_6_18_1
  doi: 10.1186/s13007-015-0046-x
– ident: e_1_2_6_33_1
  doi: 10.1163/15685411-00003184
– ident: e_1_2_6_21_1
  doi: 10.1007/s00374-016-1173-x
– ident: e_1_2_6_7_1
  doi: 10.1007/s10681-012-0686-0
– ident: e_1_2_6_14_1
  doi: 10.2134/agronj1988.00021962008000020029x
– ident: e_1_2_6_6_1
  doi: 10.1186/1746-4811-9-3
– ident: e_1_2_6_4_1
  doi: 10.1007/s11104-005-4887-3
– start-page: 73
  volume-title: Working with rhizobia
  year: 2016
  ident: e_1_2_6_32_1
  contributor:
    fullname: Yates R. J.
– ident: e_1_2_6_13_1
  doi: 10.1186/s13007-016-0131-9
– ident: e_1_2_6_20_1
  doi: 10.2307/2445879
– ident: e_1_2_6_19_1
  doi: 10.1080/17550874.2010.517788
– ident: e_1_2_6_25_1
  doi: 10.1105/tpc.114.124032
– ident: e_1_2_6_11_1
  doi: 10.1007/BF02257535
– ident: e_1_2_6_23_1
  doi: 10.1038/nmeth.2019
– ident: e_1_2_6_5_1
  doi: 10.1186/s12870-019-1791-1
– ident: e_1_2_6_9_1
  doi: 10.1093/jxb/erp083
– ident: e_1_2_6_17_1
  doi: 10.1186/1746-4811-9-38
– ident: e_1_2_6_24_1
  doi: 10.1080/03650340.2017.1342034
– ident: e_1_2_6_26_1
  doi: 10.1007/s11104-010-0623-8
– volume-title: Medicago truncatula handbook
  year: 2006
  ident: e_1_2_6_8_1
  contributor:
    fullname: Garcia J.
– ident: e_1_2_6_31_1
  doi: 10.1093/aob/mcu009
– volume-title: R: A language and environment for statistical computing
  year: 2018
  ident: e_1_2_6_22_1
  contributor:
    fullname: R Core Team
– volume-title: Medicago truncatula handbook
  year: 2006
  ident: e_1_2_6_3_1
  contributor:
    fullname: Barker D. G.
– ident: e_1_2_6_29_1
  doi: 10.1016/j.soilbio.2012.05.020
– ident: e_1_2_6_15_1
– ident: e_1_2_6_16_1
  doi: 10.1104/pp.111.179895
– ident: e_1_2_6_28_1
  doi: 10.1007/978-3-642-22067-8_17
SSID ssj0000816894
Score 2.231722
Snippet Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to...
We developed a novel low-cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to address...
PremiseWe developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to...
PREMISEWe developed a novel low-cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to...
Premise We developed a novel low‐cost method to visually phenotype belowground structures in the plant rhizosphere. We devised the method introduced here to...
SourceID doaj
pubmedcentral
proquest
crossref
pubmed
wiley
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage e11340
SubjectTerms Farms
Laboratories
Legumes
Medicago
Methods
mini‐rhizotron
Phenotypes
Plant resistance
Plant sciences
Protocol Note
Protocol Notes
Researchers
rhizobia
Rhizosphere
root phenotype
Seed germination
Seeds
symbiosis
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1bi9UwEB5k2QdfxPXa3VUi-iSUbZO0TR734rIIiqAH9i3kioK0h20X8c2f4G_0lziTnj2cg4u--FaaENKZJPPNZPoNwCshEGTY2petdqKUoU6l8laWKcQuBemsyj_SvnvfXizk28vmcqPUF-WEzfTAs-COpHWSO934xmupq2Rjq9pYKem8VtqnfPpWesOZymcwlZPQcs1Hyo_schRUwISiHBsWKBP134Yu_0yS3ASv2fqc34d7K9jIjufp7sGd2D-A3ZMBod33h7DIF__MUwkItkQ8jL3Y6RnzaKJGZkf2dfj268dPP4wTIy4RfL6iXDsKg49sGhgleg0UjWUIpCdGdSOm8REszt98Or0oV_USSt9I9AKDtaEOVRd1nZo6BHQdlGt942rnPOKAyAPab2t1Uq5rAjGJOVG3HjFiFYkY8THs9EMfnwJLynoRuBWuclJ7pyoXY-twXHS-deIFvLyRoVnOtBhmJkDmhiRtsqQLOCHxrnsQlXV-gQo2KwWbfym4gMMb5ZjV_hoNF4gMieiH396cb3_x-NIFvFg348ah2xDbx-E6D9EqBLNaFPBkVvV6ooKLpkbbXUC3tQi2vmS7pf_yOZNzd1R_QLcFvM7L5S_SMccfPor8tP8_5HQAdznFAnJW0SHsTFfX8RkCpsk9z3vjN3LIFsE
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: AUTh Library subscriptions: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NbtQwEB7BlgMXxD-BgozghBQ1sZ3EPqFuaVUhUVXASr1F_ktBQsmySYW48Qg8I0_CjDcbuqLqLYotKx6PPd-MJ98AvBYCQYbJXVpqK1Lp8yZVzsi08aFqvLRGxR9pP5yUxwv5_qw4GwNu_ZhWuTkT40HtO0cx8j0uEAsQtQt_u_yeUtUoul0dS2jchB2OnkI2g5354cnpxynKEstKaDnxkvI9s-wFFTKhaMclSxQJ-69Cmf8nS14GsdEKHd2FOyN8ZPvr9b4HN0J7H27NO4R4Px_AIiYAMEelINgScTH2YgfvmENT1TPTs2_djz-_fruuHxhxiuDzinLuKBzes6FjlPDVUVSWIaAeGNWPGPqHsDg6_HxwnI51E1JXSPQGvTE-91kVdN4UuffoQihbusLm1jrEA4F7tOPG6EbZqvDEKGZFXjrEilkggsRHMGu7NjwB1ijjhOdG2MxK7azKbAilxXHRCdcNT-DVRob1ck2PUa-JkHlNkq6jpBOYk3inHkRpHV90q_N63CG1NFZyqwtXOC111phQqjJkSlqnlXZNArubxanHfdbX_7Ti6uZ4C4zHmE7g5dSMG4huRUwbuos4RKkQ1GqRwOP1Uk8fKrgocrThCVRbSrA1k-2W9uuXSNJdUR0CXSbwJqrLNdKp908_ifj09PopPoPbnLz9mDe0C7NhdRGeIyQa7ItR7_8CNEgO_Q
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals: Open Access
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NbtQwEB5VhQMXxD-BgozghBRIbCexDwi1hapCAiHBSr1Z_ktBqpJlkwp64xF4Rp6EGW921RWr3qzYseLx2PONPfkG4IUQCDJs6fNaO5HLULa58lbmbYhNG6SzKv1I-_FTfTyTH06qkx1Y5e-cBDhsde0on9Rscfbq14-Lt7jg30wEoq_tfBCUm0Si636NS_TQKYRvgvlpR6bkElqu2Ukvv7JhjxJt_zas-X_I5GUom2zR0S24OYFItr-c9duwE7s7cP2gR6B3cRdmKQyAeUoIweaIjrEVO3zHPBqsgdmBnfU___7-4_thZMQsguUFRd7RofjAxp5R2FdPZ7MMYfXIKIvEONyD2dH7r4fH-ZQ9IfeVRJ8wWBvKUDRRl21VhoCOhHK1r1zpnEdUEHlAa26tbpVrqkC8Yk6UtUfEWESiSbwPu13fxYfAWmW9CNwKVzipvVOFi7F22C-64rrlGTxfydDMlyQZZkmHzA1J2iRJZ3BA4l23IGLr9KBfnJppnRhpneROV77yWuqitbFWdSyUdF4r7dsM9laTY1bKYrhAnEi0P3x7dboLxs1MZ_BsXY3LiO5GbBf789RFrRDaapHBg-VUrz9UcFGVaMkzaDaUYGMkmzXd92-JqruhbAS6zuBlUpcrpGP2P38RqfTo6iE-hhucfP4UPbQHu-PiPD5BYDS6p0nr_wEWGQ-L
  priority: 102
  providerName: Scholars Portal
– databaseName: Wiley Open Access
  dbid: 24P
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV3ditUwEB6W1QtvxH-rq0T0SijbJmmbgDe7q8siKAt6YO9C_qqCtIfTLsve-Qg-o0_iTHpO14OL4F1o0tBOMpkvk8k3AK-EQJBhS5_X2olchrLNlbcyb0Ns2iCdVeki7YeP9clCvj-rznbgzeYuzMQPMTvcSDPSek0Kbt2wf0UaapeDoIwkEjfsN4gyhpjzuTydPSwppUTKhMixkCN2KWZ-Ur5_9fqWRUrE_dehzb-DJv8Es8kaHd-B22sYyQ6mcb8LO7G7BzcPe4R6l_dhkQIBmKeUEGyJ-BhbsaO3zKPJGpgd2Pf-4tePn74fRkbcIlheUewducUHNvaMAr968s4yBNYjozwS4_AAFsfvPh-d5Ov8CbmvJO4Kg7WhDEUTddlWZQi4lVCu9pUrnfOICyIPaM-t1a1yTRWIWcyJsvaIGYtIRIkPYbfru_gYWKusF4Fb4QontXeqcDHWDvvFzbhueQYvNzI0y4kmw0yEyNyQpE2SdAaHJN65BVFbpwf96otZa4qR1knudOUrr6UuWhtrVcdCSee10r7NYG8zOGatb4PhApEiEf_w66vTaTAuZzqDF3M1KhKdjtgu9uepi1ohuNUig0fTUM8fKrioSrTlGTRbk2DrT7Zrum9fE1l3Q_kIdJ3B6zRd_iEdc3D6SaTSk_9p_BRucfIBpGiiPdgdV-fxGQKl0T1P-vAbaZoQEQ
  priority: 102
  providerName: Wiley-Blackwell
Title Using clear plastic CD cases as low‐cost mini‐rhizotrons to phenotype root traits
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Faps3.11340
https://www.ncbi.nlm.nih.gov/pubmed/32351801
https://www.proquest.com/docview/2395171692
https://www.proquest.com/docview/2458473079
https://search.proquest.com/docview/2396856693
https://pubmed.ncbi.nlm.nih.gov/PMC7186896
https://doaj.org/article/4ab42b95c5c9490fae686e084bc989cf
Volume 8
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NbtQwEB51CwcuiH8CZWUEJ6R0E9tJ7GN3aVUhbbUCVuot8l9KpTZZbVIhbjwCz8iTMPYmq66oOHCJrNhynPHY_sYefwPwnjEEGSo1cS41i7lNq1gYxePKuqKyXCsRLtLOz_LTJf90np3vQTbchQlO-0ZfHtZX14f15bfgW7m6NpPBT2yymM8Kz_Eu88kIRqigt0z0MP36SBKSb6lI6UStWuZjl3Af9o1RlqWijwEzrEOBrv8ujPm3q-RtCBvWoJNH8LAHj-Ro08jHsOfqJ3B_2iDA-_EUluH4nxgfCIKsEBVjKTL7SAwuVC1RLblqvv_--cs0bUc8owim197jzm-Gt6RriHf3avyeLEE43REfPaJrn8Hy5Pjr7DTuoybEJuNoC1qlbGqTwsm0ylJr0YAQOjeZTrU2iAYctbiKKyUroYvMej4xzdLcIFJMnKdHfA77dVO7l0AqoQyzVDGdaC6NFol2LtdYL5rgsqIRvBtkWK425BjlhgaZll7oZRB6BFMv3m0JT2gdXjTri7Lv1pIrzamWmcmM5DKplMtF7hLBtZFCmiqCg6Fzyn6UtSVliA893Q-9OzucAeMkJiN4u83G4ePPRFTtmptQRS4Q0koWwYtNV28bOqhKBMWOEuz8yW4Oamyg6O41NIIPQV3-IZ3yaPGFhdSr__7Ma3hA_TZAcCg6gP1ufePeIFbq9BhGlC_GcG96fLb4PA47DvicczEOo-YPl2oa4Q
link.rule.ids 230,315,730,783,787,867,888,2109,2228,11574,21400,24330,27936,27937,33756,33757,43817,46064,46488,50826,50935,53804,53806,74630
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3NbtQwELagRYIL4rcEChjBCSlqYjuJfaq6pdUC7aqCrtSb5b8AEkqWTSrEjUfgGfskzHizS1dUvUWxZcVjj-eb8eQbQt5wDiDD5C4tleWp8HmdSmdEWvtQ1V5YI-OPtMeTcjwVH86KsyHg1g1plcszMR7UvnUYI99hHLAAUruw3dmPFKtG4e3qUELjJtlEqipwvjZHB5OTT6soSywrocSKl5TtmFnHsZAJRjsuWaJI2H8Vyvw_WfIyiI1W6PAeuTvAR7q3WO_75EZoHpBboxYg3q-HZBoTAKjDUhB0BrgYetH9d9SBqeqo6ej39ufF7z-u7XqKnCLwPMecOwyHd7RvKSZ8tRiVpQCoe4r1I_ruEZkeHpzuj9OhbkLqCgHeoDfG5z6rgsrrIvceXAhpS1fY3FoHeCAwD3bcGFVLWxUeGcUsz0sHWDELSJD4mGw0bROeEFpL47hnhtvMCuWszGwIpYVxwQlXNUvI66UM9WxBj6EXRMhMo6R1lHRCRijeVQ-ktI4v2vkXPWiIFsYKZlXhCqeEymoTSlmGTArrlFSuTsj2cnH0oGed_rcrrm6Ot8BwjKmEvFo1gwLhrYhpQnsehyglgFrFE7K1WOrVh3LGixxseEKqtU2wNpP1lubb10jSXWEdAlUm5G3cLtdIR--dfObx6en1U3xJbo9Pj4_00fvJx2fkDkPPP-YQbZONfn4engM86u2LQQf-Aji4Efc
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB7BFiEuiGdJKWAEJ6Rok9h5-IS6bVfltVoBK_UW-RVAQsl2kwpx4yfwG_klzHizoSuq3qLYsuLxjOfzePINwEvOEWSo2ISZ1DwUNq7CwigRVtbllRVaFf5H2g-z7GQh3p6mp33-U9unVW72RL9R28ZQjHyccMQCRO2SjKs-LWJ-NH29PAupghTdtPblNK7DTi4yHo1gZ3I8m38cIi6-xIQUA0dpMlbLllNRE4p8XPBKnrz_MsT5f-LkRUDrPdL0DtzuoSQ7WK_9Xbjm6ntwY9Ig3Pt5HxY-GYAZKgvBloiRsRc7PGIG3VbLVMu-Nz_-_PptmrZjxC-CzyvKv6PQeMu6hlHyV0MRWobgumNUS6JrH8Bievz58CTsayiEJhV4MrRK2dhGuZNxlcbW4nGi0JlJday1QWzgEos-XSlZFTpPLbGLaR5nBnFj5Igs8SGM6qZ2j4BVhTLcJorrSAtpdBFp5zKN4-KBXFZJAC82MiyXa6qMck2KnJQk6dJLOoAJiXfoQfTW_kWz-lL21lIKpUWiZWpSI4WMKuWyInNRIbSRhTRVAPubxSl7m2vLfxpyebO_EcYtTQbwfGhGY6IbElW75twPkRUIcCUPYHe91MOH8oSnMfrzAPItJdiayXZL_e2rJ-zOqSaBzAJ45dXlCumUB_NP3D_tXT3FZ3AT1b98_2b27jHcSigI4NOJ9mHUrc7dE0RKnX7am8Bf2hwWJQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Using+clear+plastic+CD+cases+as+low%E2%80%90cost+mini%E2%80%90rhizotrons+to+phenotype+root+traits&rft.jtitle=Applications+in+plant+sciences&rft.au=Cassidy%2C+Steven+T&rft.au=Burr%2C+Audrey+A&rft.au=Reeb%2C+Rachel+A&rft.au=Melero+Pardo%2C+Ana+L&rft.date=2020-04-01&rft.pub=John+Wiley+%26+Sons%2C+Inc&rft.eissn=2168-0450&rft.volume=8&rft.issue=4&rft_id=info:doi/10.1002%2Faps3.11340&rft.externalDBID=HAS_PDF_LINK
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2168-0450&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2168-0450&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2168-0450&client=summon