Enzymatic Regioselection for the Synthesis and Biodegradation of Porphysome Nanovesicles

• Published in: Angewandte Chemie International Edition Vol. 51; no. 10; pp. 2429 - 2433
• Main Authors: Lovell, Jonathan F., Jin, Cheng S., Huynh, Elizabeth, MacDonald, Thomas D., Cao, Weiguo, Zheng, Gang
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 05.03.2012; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Animals; Biodegradation; Chromatography, High Pressure Liquid; Conjugates; Enzymes; Enzymes - chemistry; Isomerism; Liver; Magnetic Resonance Spectroscopy; Mice; Microscopy, Electron, Transmission; nanoparticles; Nanoparticles - chemistry; Nanostructure; phospholipids; Phospholipids - chemistry; Phospholipids - metabolism; porphyrinoids; Porphyrins - chemistry; Porphyrins - metabolism; regioselectivity; Screens; Serums; Stereoisomerism; Synthesis; Uptakes

A select few: An enzyme screen is used to identify two enzymes that selectively cleave porphyrin–lipid regioisomers (see picture). Either of the isomerically pure conjugates assembles into physically similar porphysome nanovesicles. Although porphysomes formed from both regioisomers are stable in se...