Global Transcriptional Start Site Mapping Using Differential RNA Sequencing Reveals Novel Antisense RNAs in Escherichia coli

• Published in: Journal of bacteriology Vol. 197; no. 1; pp. 18 - 28
• Main Authors: Thomason, Maureen K., Bischler, Thorsten, Eisenbart, Sara K., Förstner, Konrad U., Zhang, Aixia, Herbig, Alexander, Nieselt, Kay, Sharma, Cynthia M., Storz, Gisela
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.01.2015
• Subjects: Algorithms; Bacteriology; Chromosome Mapping; complementary DNA; Deoxyribonucleic acid; DNA; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Gene expression; Gene Expression Regulation, Bacterial - physiology; genes; Genetics; Genome, Bacterial; Genomics; Growth conditions; mutation; prediction; ribonucleases; Ribonucleic acid; RNA; RNA, Antisense - genetics; RNA, Antisense - metabolism; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; Sequence Analysis, RNA - methods; surveys; transcription (genetics); Transcription Initiation Site - physiology; Transcriptome

While the model organism Escherichia coli has been the subject of intense study for decades, the full complement of its RNAs is only now being examined. Here we describe a survey of the E. coli transcriptome carried out using a differential RNA sequencing (dRNA-seq) approach, which can distinguish b...