Screening and identification of evaluation indicators of low phosphorus tolerant germplasm in Gleditsia sinensis Lam

• Published in: Scientific reports Vol. 14; no. 1; pp. 31716 - 16
• Main Authors: Lu, Chunyun, Wang, Xiurong, Zhao, Yang, Zou, Rong, Xiao, Feng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.12.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/443; 631/449; Biomass; Dry weight; Evaluation; Germplasm; Germplasm resources; Gleditsia sinensis; Gleditsia sinensis Lam; Humanities and Social Sciences; Lamiaceae - drug effects; Lamiaceae - growth & development; Lamiaceae - metabolism; Lamiaceae - physiology; Leaves; Low phosphorus stress; Morphology; multidisciplinary; Phosphorus; Phosphorus - metabolism; Plant Roots - drug effects; Plant Roots - growth & development; Plant Roots - metabolism; Principal components analysis; Regression analysis; Science; Science (multidisciplinary); Screening evaluation; Sensitivity analysis; Shoots; Low phosphorus stress; Lam; Screening evaluation; Germplasm resources; Gleditsia sinensis Lam
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-82071-w

This study aims to explore the low phosphorus (P) tolerance of saplings from different Gleditsia sinensis Lam. families. It also seeks to screen for Gleditsia sinensis families with strong low P tolerance and identify key indicators for evaluating their tolerance. This research provides a foundation for the breeding of superior families of Gleditsia sinensis and the study of mechanisms underlying low P tolerance. Using saplings from 30 Gleditsia sinensis families as the research subjects, a sand culture pot experiment was conducted. This study set up low P treatment (0.01 mmol L −1 ) and normal P treatment (1 mmol L −1 ). Twenty-five indicators including growth morphology, biomass, root morphology, and P content were measured. The low P tolerance coefficient was used as the basic data for assessing the low P tolerance of Gleditsia sinensis . The fuzzy comprehensive evaluation method was employed to comprehensively assess the low P tolerance types of Gleditsia sinensis a stepwise regression model was established to identify the key evaluation indicators for low P tolerance. The results indicate that low P stress reduced plant height, stem diameter, and biomass in most Gleditsia sinensis families, but increased the root morphological indicators, root-shoot ratio and PUE of various organs. Principal component analysis transformed the 25 indicators into 6 independent comprehensive indicators, with a cumulative contribution rate of 86.743%. The fuzzy comprehensive evaluation method calculated a comprehensive evaluation value (D value), enabling the screening of Gleditsia sinensis families into low P tolerant and low P sensitive types. Cluster analysis grouped the 30 Gleditsia sinensis families into 4 types. Among them, F13, F10, F9, F18, F15, and F28 were classified as low P tolerant types; F6, F23, F3, F17, F20, F2, F12, F11, F16, F8, F5, F27, F1, and F26 were categorized as intermediate types; F30, F7, F22, F4, F19, F29, F24, F14 and F25 were considered low P sensitive types, and F21 was classified as extremely low P sensitive types. The stepwise regression analysis identified the indicators stem diameter, total root volume, shoot dry weight, total root projection area, and leaf P content as the key factors for discriminating the low P tolerance of Gleditsia sinensis . The regression model is as follows: D=-0.005 + 0.323 stem diameter *+0.154 * total root volume + 0.196* shoot dry weight + 0.139* total root projection area − 0.112* leaf P content. In summary, low P stress inhibited the growth of Gleditsia sinensis saplings, but it increased the root morphological indicators, root-shoot ratio and PUE of various organs to cope with low P environments. The screening identified F13, F10, F9, F18, F15, and F28 as low P tolerant Gleditsia sinensis families. The evaluation indicators for low P tolerance in Gleditsia sinensis were identified as stem diameter, total root volume, shoot dry weight, total root projection area and leaf P content.