LC-MS Profiling of Kakkonto and Identification of Ephedrine as a Key Component for Its Anti-Glycation Activity
A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids,...
Saved in:
| Published in | Molecules Vol. 28; no. 11; p. 4409 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Switzerland
MDPI AG
29.05.2023
MDPI |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids, fourteen flavonoids, two but-2-enolides, five monoterpenoids, and four triterpenoid glycosides. To identify the components responsible for its anti-glycation activity, the Kakkonto extract was reacted with glyceraldehyde (GA) or methylglyoxal (MGO) and analyzed using LC-MS. In LC-MS analysis of Kakkonto reacted with GA, the peak intensity of ephedrine was attenuated, and three products from ephedrine-scavenging GA were detected. Similarly, LC-MS analysis of Kakkonto reacted with MGO revealed two products from ephedrine reacting with MGO. These results indicated that ephedrine was responsible for the observed anti-glycation activity of Kakkonto. Ephedrae herba extract, which contains ephedrine, also showed strong anti-glycation activity, further supporting ephedrine’s contribution to Kakkonto’s reactive carbonyl species’ scavenging ability and anti-glycation activity. |
|---|---|
| AbstractList | A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids, fourteen flavonoids, two but-2-enolides, five monoterpenoids, and four triterpenoid glycosides. To identify the components responsible for its anti-glycation activity, the Kakkonto extract was reacted with glyceraldehyde (GA) or methylglyoxal (MGO) and analyzed using LC-MS. In LC-MS analysis of Kakkonto reacted with GA, the peak intensity of ephedrine was attenuated, and three products from ephedrine-scavenging GA were detected. Similarly, LC-MS analysis of Kakkonto reacted with MGO revealed two products from ephedrine reacting with MGO. These results indicated that ephedrine was responsible for the observed anti-glycation activity of Kakkonto. Ephedrae herba extract, which contains ephedrine, also showed strong anti-glycation activity, further supporting ephedrine's contribution to Kakkonto's reactive carbonyl species' scavenging ability and anti-glycation activity.A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids, fourteen flavonoids, two but-2-enolides, five monoterpenoids, and four triterpenoid glycosides. To identify the components responsible for its anti-glycation activity, the Kakkonto extract was reacted with glyceraldehyde (GA) or methylglyoxal (MGO) and analyzed using LC-MS. In LC-MS analysis of Kakkonto reacted with GA, the peak intensity of ephedrine was attenuated, and three products from ephedrine-scavenging GA were detected. Similarly, LC-MS analysis of Kakkonto reacted with MGO revealed two products from ephedrine reacting with MGO. These results indicated that ephedrine was responsible for the observed anti-glycation activity of Kakkonto. Ephedrae herba extract, which contains ephedrine, also showed strong anti-glycation activity, further supporting ephedrine's contribution to Kakkonto's reactive carbonyl species' scavenging ability and anti-glycation activity. A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated significant anti-glycation activity, prompting further analysis of its chemical constituents using LC-MS, which revealed the presence of two alkaloids, fourteen flavonoids, two but-2-enolides, five monoterpenoids, and four triterpenoid glycosides. To identify the components responsible for its anti-glycation activity, the Kakkonto extract was reacted with glyceraldehyde (GA) or methylglyoxal (MGO) and analyzed using LC-MS. In LC-MS analysis of Kakkonto reacted with GA, the peak intensity of ephedrine was attenuated, and three products from ephedrine-scavenging GA were detected. Similarly, LC-MS analysis of Kakkonto reacted with MGO revealed two products from ephedrine reacting with MGO. These results indicated that ephedrine was responsible for the observed anti-glycation activity of Kakkonto. Ephedrae herba extract, which contains ephedrine, also showed strong anti-glycation activity, further supporting ephedrine’s contribution to Kakkonto’s reactive carbonyl species’ scavenging ability and anti-glycation activity. |
| Author | Kanako Ikube Kouharu Otsuki Takashi Kikuchi Kazuo Koike Kaori Ito Wei Li |
| AuthorAffiliation | Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan |
| AuthorAffiliation_xml | – name: Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, Japan |
| Author_xml | – sequence: 1 givenname: Kaori surname: Ito fullname: Ito, Kaori – sequence: 2 givenname: Takashi orcidid: 0000-0001-8832-5720 surname: Kikuchi fullname: Kikuchi, Takashi – sequence: 3 givenname: Kanako surname: Ikube fullname: Ikube, Kanako – sequence: 4 givenname: Kouharu orcidid: 0000-0001-6730-0663 surname: Otsuki fullname: Otsuki, Kouharu – sequence: 5 givenname: Kazuo surname: Koike fullname: Koike, Kazuo – sequence: 6 givenname: Wei orcidid: 0000-0003-4143-8597 surname: Li fullname: Li, Wei |
| BackLink | https://cir.nii.ac.jp/crid/1870302168130690048$$DView record in CiNii https://www.ncbi.nlm.nih.gov/pubmed/37298887$$D View this record in MEDLINE/PubMed |
| BookMark | eNplkl9vFCEUxSemxtbaD-CLIdEHX0Yv_-HJbDa1brpGE_WZMAxsaWdhZWab7LeXdtem1Rcg3N85HOC-bI5STr5pXmP4QKmGj-s8eLcd_EgUxoyBftacYEagpcD00aP1cXM2jrEDxilhUsOL5phKopVS8qRJy3n79Qf6XnKIQ0wrlAO6tDc3OU0Z2dSjRe_TFEN0doo53ZXPN1e-LzF5ZEdk0aXfoXleb2q6NKGQC1pMI5pVUXsx7A6ymZvibZx2r5rnwQ6jPzvMp82vz-c_51_a5beLxXy2bB1neGoDw06BkIxJr7CXfejAc8t5T4LusNa96hQhXgbei65jAF1vLXVg-4oxQU-bxd63z_babEpc27Iz2UZzv5HLytgyRTd4IwAToYT0ARNGLVainqK4Dp2VigdVvT7tvTbbbu17V69Z7PDE9GklxSuzyrcGA-GMcF0d3h8cSv699eNk1nF0fhhs8nk7GqIIE5pKQSv69h_0Om9Lqm91TwFwIaBSbx5Hesjy918rgPeAK3kciw8PCAZz1z7mv_apmnd7TYrRuHg3YiWBAsFCYQpCAzBF_wBQt8Ne |
| Cites_doi | 10.1074/jbc.271.32.19338 10.1021/acs.jnatprod.5b00877 10.1021/acs.jafc.1c03582 10.1007/BF02978244 10.1016/j.fitote.2015.11.011 10.1016/j.abb.2003.08.013 10.1007/s00216-010-4065-1 10.1021/acs.analchem.6b04919 10.1016/0031-9422(93)85050-2 10.3390/nu14112338 10.3389/fphar.2021.687818 10.3390/molecules20046273 10.1007/s11418-019-01329-0 10.2337/diabetes.48.1.198 10.1016/j.bioorg.2018.10.017 10.1021/pr800858h 10.1002/rcm.6696 10.1016/j.jpba.2016.07.031 10.1002/mas.20090 10.1002/anie.201810037 10.3390/molecules27196227 10.1002/jssc.201400522 10.1186/1472-6882-14-64 10.1039/C6AY00732E 10.1016/0040-4020(77)80156-7 10.1007/s11418-018-1259-x 10.2337/dc15-2727 10.1016/S0531-5565(01)00138-3 10.1080/10286020802016321 10.1186/1472-6882-14-435 10.1002/jms.842 10.1016/S0197-2456(99)00024-0 10.1007/s11418-019-01327-2 10.1007/s11418-020-01474-x 10.1016/j.cardiores.2004.05.001 10.1007/s12272-012-1111-x 10.1016/S1262-3636(03)72793-1 10.2174/1567205043480582 10.1007/s11418-021-01566-2 10.1007/s001250051591 10.1142/S0192415X01000514 10.3892/etm.2012.462 10.1016/j.jep.2016.03.063 10.1016/j.jpba.2012.09.015 |
| ContentType | Journal Article |
| Copyright | 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2023 by the authors. 2023 |
| Copyright_xml | – notice: 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2023 by the authors. 2023 |
| DBID | RYH AAYXX CITATION NPM 3V. 7X7 7XB 88E 8FI 8FJ 8FK ABUWG AFKRA AZQEC BENPR CCPQU DWQXO FYUFA GHDGH K9. M0S M1P PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS 7X8 5PM DOA |
| DOI | 10.3390/molecules28114409 |
| DatabaseName | CiNii Complete CrossRef PubMed ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Hospital Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central ProQuest One Community College ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Health & Medical Complete (Alumni) ProQuest Health & Medical Collection PML(ProQuest Medical Library) ProQuest Central Premium ProQuest One Academic Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef PubMed Publicly Available Content Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Central China ProQuest Central ProQuest Health & Medical Research Collection Health Research Premium Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Health & Medical Research Collection ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Publicly Available Content Database PubMed CrossRef |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Chemistry |
| EISSN | 1420-3049 |
| ExternalDocumentID | oai_doaj_org_article_60126867ef1243a1865d2859fba785f8 PMC10254259 37298887 10_3390_molecules28114409 |
| Genre | Journal Article |
| GeographicLocations | Japan |
| GeographicLocations_xml | – name: Japan |
| GroupedDBID | --- 0R~ 123 2WC 53G 5VS 7X7 88E 8FE 8FG 8FH 8FI 8FJ A8Z AADQD AAFWJ AAHBH ABDBF ABUWG ACGFO ACIWK ACPRK ACUHS AEGXH AENEX AFKRA AFPKN AFRAH AFZYC AIAGR ALIPV ALMA_UNASSIGNED_HOLDINGS BENPR BPHCQ BVXVI CCPQU CS3 D1I DIK DU5 E3Z EBD EMOBN ESX FYUFA GROUPED_DOAJ GX1 HH5 HMCUK HYE HZ~ I09 IAO IHR ITC KQ8 LK8 M1P MODMG O-U O9- OK1 P2P PHGZM PHGZT PIMPY PQQKQ PROAC PSQYO RPM RYH SV3 TR2 TUS UKHRP ~8M AAYXX CITATION 3V. ABJCF BBNVY BHPHI HCIFZ KB. M7P M~E NPM PDBOC 7XB 8FK AZQEC DWQXO K9. PJZUB PKEHL PPXIY PQEST PQUKI PRINS 7X8 PUEGO 5PM |
| ID | FETCH-LOGICAL-c541t-f41c8067447e81e7dfb0e5a55d2f9b199d8b822e7f5d6bb400bdaa3c0ade5a463 |
| IEDL.DBID | DOA |
| ISSN | 1420-3049 |
| IngestDate | Wed Aug 27 01:29:12 EDT 2025 Thu Aug 21 18:38:00 EDT 2025 Sun Aug 24 04:13:06 EDT 2025 Fri Jul 25 19:58:28 EDT 2025 Wed Feb 19 02:23:38 EST 2025 Tue Jul 01 01:22:09 EDT 2025 Thu Jun 26 23:36:11 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 11 |
| Keywords | Kampo ephedrine Kakkonto advanced glycation end products anti-glycation activity |
| Language | English |
| License | https://creativecommons.org/licenses/by/4.0 Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c541t-f41c8067447e81e7dfb0e5a55d2f9b199d8b822e7f5d6bb400bdaa3c0ade5a463 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| ORCID | 0000-0001-6730-0663 0000-0003-4143-8597 0000-0001-8832-5720 |
| OpenAccessLink | https://doaj.org/article/60126867ef1243a1865d2859fba785f8 |
| PMID | 37298887 |
| PQID | 2824005660 |
| PQPubID | 2032355 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_60126867ef1243a1865d2859fba785f8 pubmedcentral_primary_oai_pubmedcentral_nih_gov_10254259 proquest_miscellaneous_2824693763 proquest_journals_2824005660 pubmed_primary_37298887 crossref_primary_10_3390_molecules28114409 nii_cinii_1870302168130690048 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2023-05-29 |
| PublicationDateYYYYMMDD | 2023-05-29 |
| PublicationDate_xml | – month: 05 year: 2023 text: 2023-05-29 day: 29 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland – name: Basel |
| PublicationTitle | Molecules |
| PublicationTitleAlternate | Molecules |
| PublicationYear | 2023 |
| Publisher | MDPI AG MDPI |
| Publisher_xml | – name: MDPI AG – name: MDPI |
| References | Lee (ref_34) 1994; 17 Welsh (ref_1) 2016; 39 Cheng (ref_28) 2019; 73 Ji (ref_33) 2016; 79 Sun (ref_36) 2008; 10 Singh (ref_7) 2001; 44 Tsuge (ref_15) 2022; 76 Freedman (ref_14) 1999; 20 ref_35 Beisswenger (ref_13) 1999; 48 Onoda (ref_21) 2014; 14 Huang (ref_27) 2012; 35 Lapolla (ref_3) 2006; 25 Shi (ref_26) 2016; 129 ref_18 Xiu (ref_42) 2001; 29 Nohara (ref_38) 1993; 33 Takiyama (ref_17) 2021; 75 Shi (ref_39) 2016; 108 Beisswenger (ref_12) 2003; 29 Basta (ref_10) 2004; 63 Lapolla (ref_2) 2005; 40 Onoda (ref_20) 2016; 186 Zhang (ref_4) 2009; 8 Miao (ref_24) 2013; 72 Xu (ref_30) 2013; 27 Song (ref_44) 2012; 3 Nagaraj (ref_6) 1996; 271 Derbre (ref_22) 2010; 398 Sato (ref_19) 2019; 73 Zheng (ref_40) 2015; 20 ref_43 Takeuchi (ref_9) 2004; 1 Tao (ref_25) 2016; 8 Toume (ref_16) 2019; 73 Saito (ref_23) 2021; 12 Ahn (ref_37) 2019; 83 Sjoblom (ref_5) 2018; 57 Song (ref_31) 2017; 89 Beckett (ref_41) 1977; 33 Kim (ref_29) 2014; 37 Zhang (ref_32) 2021; 69 Baynes (ref_8) 2001; 36 Thornalley (ref_11) 2003; 419 |
| References_xml | – volume: 271 start-page: 19338 year: 1996 ident: ref_6 article-title: Protein crosslinking by the Maillard reaction. Isolation, characterization, and in vivo detection of a lysine-lysine cross-link derived from methylglyoxal publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.32.19338 – volume: 79 start-page: 281 year: 2016 ident: ref_33 article-title: Bioactive Constituents of Glycyrrhiza uralensis (Licorice): Discovery of the Effective Components of a Traditional Herbal Medicine publication-title: J. Nat. Prod. doi: 10.1021/acs.jnatprod.5b00877 – volume: 69 start-page: 10943 year: 2021 ident: ref_32 article-title: Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis publication-title: J. Agric. Food Chem. doi: 10.1021/acs.jafc.1c03582 – volume: 17 start-page: 31 year: 1994 ident: ref_34 article-title: Antiinflammatory activity of isoflavonoids from Pueraria radix and biochanin A derivatives publication-title: Arch. Pharmacal Res. doi: 10.1007/BF02978244 – volume: 108 start-page: 55 year: 2016 ident: ref_39 article-title: Monoterpene derivatives with anti-allergic activity from red peony root, the root of Paeonia lactiflora publication-title: Fitoterapia doi: 10.1016/j.fitote.2015.11.011 – volume: 419 start-page: 31 year: 2003 ident: ref_11 article-title: Use of aminoguanidine (Pimagedine) to prevent the formation of advanced glycation endproducts publication-title: Arch. Biochem. Biophys. doi: 10.1016/j.abb.2003.08.013 – volume: 398 start-page: 1747 year: 2010 ident: ref_22 article-title: Automating a 96-well microtiter plate assay for identification of AGEs inhibitors or inducers: Application to the screening of a small natural compounds library publication-title: Anal. Bioanal. Chem. doi: 10.1007/s00216-010-4065-1 – volume: 89 start-page: 3146 year: 2017 ident: ref_31 article-title: Biosynthesis-Based Quantitative Analysis of 151 Secondary Metabolites of Licorice To Differentiate Medicinal Glycyrrhiza Species and Their Hybrids publication-title: Anal. Chem. doi: 10.1021/acs.analchem.6b04919 – volume: 33 start-page: 1207 year: 1993 ident: ref_38 article-title: But-2-enolides from Pueraria lobata and revised structures of puerosides A, B and sophoroside A publication-title: Phytochemistry doi: 10.1016/0031-9422(93)85050-2 – ident: ref_43 doi: 10.3390/nu14112338 – volume: 12 start-page: 687818 year: 2021 ident: ref_23 article-title: Kakkonto inhibits cytokine production induced by rhinovirus infection in primary cultures of human nasal epithelial cells publication-title: Front. Pharmacol. doi: 10.3389/fphar.2021.687818 – volume: 20 start-page: 6273 year: 2015 ident: ref_40 article-title: Hepatoprotective triterpene saponins from the roots of Glycyrrhiza inflata publication-title: Molecules doi: 10.3390/molecules20046273 – volume: 73 start-page: 847 year: 2019 ident: ref_28 article-title: Isolation, structural elucidation and in vitro hepatoprotective activity of flavonoids from Glycyrrhiza uralensis publication-title: J. Nat. Med. doi: 10.1007/s11418-019-01329-0 – volume: 48 start-page: 198 year: 1999 ident: ref_13 article-title: Metformin reduces systemic methylglyoxal levels in type 2 diabetes publication-title: Diabetes doi: 10.2337/diabetes.48.1.198 – volume: 83 start-page: 135 year: 2019 ident: ref_37 article-title: Dual effects of isoflavonoids from Pueraria lobata roots on estrogenic activity and anti-proliferation of MCF-7 human breast carcinoma cells publication-title: Bioorg. Chem. doi: 10.1016/j.bioorg.2018.10.017 – volume: 8 start-page: 754 year: 2009 ident: ref_4 article-title: A Perspective on the maillard reaction and the analysis of protein glycation by mass spectrometry: Probing the pathogenesis of chronic disease publication-title: J. Proteome Res. doi: 10.1021/pr800858h – volume: 27 start-page: 2297 year: 2013 ident: ref_30 article-title: An integrated exact mass spectrometric strategy for comprehensive and rapid characterization of phenolic compounds in licorice publication-title: Rapid Commun. Mass Spectrom. doi: 10.1002/rcm.6696 – volume: 129 start-page: 581 year: 2016 ident: ref_26 article-title: Characterization and quantification of monoterpenoids in different types of peony root and the related Paeonia species by liquid chromatography coupled with ion trap and time-of-flight mass spectrometry publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2016.07.031 – volume: 25 start-page: 775 year: 2006 ident: ref_3 article-title: The role of mass spectrometry in the study of non-enzymatic protein glycation in diabetes: An update publication-title: Mass Spectrom. Rev. doi: 10.1002/mas.20090 – volume: 57 start-page: 16077 year: 2018 ident: ref_5 article-title: A Systematic study of selective protein glycation publication-title: Angew. Chem. Int. Ed. doi: 10.1002/anie.201810037 – ident: ref_35 doi: 10.3390/molecules27196227 – volume: 37 start-page: 2704 year: 2014 ident: ref_29 article-title: Chemical interaction between Paeonia lactiflora and Glycyrrhiza uralensis, the components of Jakyakgamcho-tang, using a validated high-performance liquid chromatography method: Herbal combination and chemical interaction in a decoction publication-title: J. Sep. Sci. doi: 10.1002/jssc.201400522 – ident: ref_18 doi: 10.1186/1472-6882-14-64 – volume: 8 start-page: 4211 year: 2016 ident: ref_25 article-title: Rapid characterization and determination of isoflavones and triterpenoid saponins in Fu-Zhu-Jiang-Tang tablets using UHPLC-Q-TOF/MS and HPLC-UV publication-title: Anal. Methods doi: 10.1039/C6AY00732E – volume: 33 start-page: 3313 year: 1977 ident: ref_41 article-title: Identification and stereochemistry of (2S,4S,5R)- and (2R,4S,5R)-2,3,4-trimethyl-5-phenyloxazolidine, degradation products of ephedrine publication-title: Tetrahedron doi: 10.1016/0040-4020(77)80156-7 – volume: 73 start-page: 163 year: 2019 ident: ref_19 article-title: Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent publication-title: J. Nat. Med. doi: 10.1007/s11418-018-1259-x – volume: 39 start-page: 1299 year: 2016 ident: ref_1 article-title: Role of glycated proteins in the diagnosis and management of diabetes: Research gaps and future directions publication-title: Diabetes Care doi: 10.2337/dc15-2727 – volume: 36 start-page: 1527 year: 2001 ident: ref_8 article-title: The role of AGEs in aging: Causation or correlation publication-title: Exp. Gerontol. doi: 10.1016/S0531-5565(01)00138-3 – volume: 10 start-page: 719 year: 2008 ident: ref_36 article-title: Two new isoflavone glycosides from Pueraria lobata publication-title: J. Asian Nat. Prod. Res. doi: 10.1080/10286020802016321 – volume: 14 start-page: 435/1 year: 2014 ident: ref_21 article-title: Inhibitory activities of selected Kampo formulations on human aldose reductase publication-title: BMC Complement. Altern. Med. doi: 10.1186/1472-6882-14-435 – volume: 40 start-page: 969 year: 2005 ident: ref_2 article-title: Mass spectrometric study of in vivo production of advanced glycation endproducts/peptides publication-title: J. Mass Spectrom. doi: 10.1002/jms.842 – volume: 20 start-page: 493 year: 1999 ident: ref_14 article-title: Design and baseline characteristics for the aminoguanidine Clinical Trial in Overt Type 2 Diabetic Nephropathy (ACTION II) publication-title: Control. Clin. Trials doi: 10.1016/S0197-2456(99)00024-0 – volume: 73 start-page: 761 year: 2019 ident: ref_16 article-title: Search of anti-allodynic compounds from Plantaginis Semen, a crude drug ingredient of Kampo formula “Goshajinkigan” publication-title: J. Nat. Med. doi: 10.1007/s11418-019-01327-2 – volume: 75 start-page: 361 year: 2021 ident: ref_17 article-title: Pharmacokinetic study of traditional Japanese Kampo medicine shimotsuto used to treat gynecological diseases in rats publication-title: J. Nat. Med. doi: 10.1007/s11418-020-01474-x – volume: 63 start-page: 582 year: 2004 ident: ref_10 article-title: Advanced glycation end products and vascular inflammation: Implications for accelerated atherosclerosis in diabetes publication-title: Cardiovasc. Res. doi: 10.1016/j.cardiores.2004.05.001 – volume: 35 start-page: 1945 year: 2012 ident: ref_27 article-title: Comparative study of bioactive constituents in crude and processed Glycyrrhizae radix and their respective metabolic profiles in gastrointestinal tract in vitro by HPLC-DAD and HPLC-ESI/MS analyses publication-title: Arch. Pharmacal Res. doi: 10.1007/s12272-012-1111-x – volume: 29 start-page: 6S95 year: 2003 ident: ref_12 article-title: Metformin inhibition of glycation processes publication-title: Diabetes Metab. doi: 10.1016/S1262-3636(03)72793-1 – volume: 1 start-page: 39 year: 2004 ident: ref_9 article-title: Involvement of advanced glycation end-products (AGEs) in Alzheimer’s disease publication-title: Curr. Alzheimer Res. doi: 10.2174/1567205043480582 – volume: 76 start-page: 144 year: 2022 ident: ref_15 article-title: Orengedokuto exerts anti-allergic effects via inhibition of effector T cell activation in a murine model of contact hypersensitivity publication-title: J. Nat. Med. doi: 10.1007/s11418-021-01566-2 – volume: 44 start-page: 129 year: 2001 ident: ref_7 article-title: Advanced glycation end-products: A review publication-title: Diabetologia doi: 10.1007/s001250051591 – volume: 29 start-page: 493 year: 2001 ident: ref_42 article-title: Pancreatic islet regeneration by ephedrine in mice with streptozotocin-induced diabetes publication-title: Am. J. Chin. Med. doi: 10.1142/S0192415X01000514 – volume: 3 start-page: 707 year: 2012 ident: ref_44 article-title: Beneficial effect of dietary Ephedra sinica on obesity and glucose intolerance in high-fat diet-fed mice publication-title: Exp. Ther. Med. doi: 10.3892/etm.2012.462 – volume: 186 start-page: 84 year: 2016 ident: ref_20 article-title: Identification and evaluation of magnolol and chrysophanol as the principle protein tyrosine phosphatase-1B inhibitory compounds in a Kampo medicine, Masiningan publication-title: J. Ethnopharmacol. doi: 10.1016/j.jep.2016.03.063 – volume: 72 start-page: 99 year: 2013 ident: ref_24 article-title: Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2012.09.015 |
| SSID | ssib045324790 ssib045324892 ssib045324860 ssib045324871 ssib045317781 ssj0021415 ssib045318462 ssib019524205 ssib045315123 ssib045323736 ssib045323679 ssib045315234 ssib045316114 ssib045323676 ssib045315933 ssib045318904 ssib045318937 ssib045318914 ssib005901244 |
| Score | 2.3814583 |
| Snippet | A total of 147 oral Kampo prescriptions, which are used clinically in Japan, were evaluated for their anti-glycation activity. Kakkonto demonstrated... |
| SourceID | doaj pubmedcentral proquest pubmed crossref nii |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 4409 |
| SubjectTerms | advanced glycation end products Alzheimer's disease anti-glycation activity Antifungal agents Chromatography, Liquid Diabetes Drugs, Chinese Herbal Ephedrine Glucose Glycation End Products, Advanced Kakkonto Kampo Kampo; Kakkonto; anti-glycation activity; advanced glycation end products; ephedrine Magnesium Oxide Organic chemistry Prescriptions Proteins Pyruvaldehyde QD241-441 Tandem Mass Spectrometry |
| SummonAdditionalLinks | – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB5BOcAF8SbQIiNxQrKaeGPHPqFl1VIoRUhQaW-Rn3RVSJZme-i_ZybJbllAXHKIbcXxN5mHx_kG4FUeovSlUtxVcihhxp2Pnuc-WROSRdgpo3vySR2dlh_mcj5uuHXjscq1TuwVdWg97ZHvY2hQEm-lyt8sf3KqGkXZ1bGExk24RdRlJNXV_DrgKtA6DZnMCYb2-z-GgrOxE7qgnKbZskU9ZT9amGax-Je3-eehyd-s0OE9uDu6j2w64H0fbsTmAdyerau2PYTm44yffGGf-1LcaJZYm9ixPT8nmgJmm8CGP3PTuFVHzQfLsxjoJ0BmO2bZcbxipCXaBjsy9GnZ-1XHpjiIv_t-NQ6b-qHqxCM4PTz4OjviY00F7mVZrHgqC6_RQpVlFXURq5BcHqWVMohkXGFM0A59hlglGZRzuOQuWDvxuUVQbakmj2GnwQk8BeZDyokOTxDaQuQaxwslTI7YJ1H6DF6vV7deDtQZNYYcBEX9FxQZvKX133Qk1uv-RnvxrR4_ohqDR6HwiTGhVzKx-HCctpYmOVtpmXQGe4he7Rd0LTQpM1EojWZaGVJVGeyuca3HT7WrrwUrg5ebZkSNMie2ie3l0EcZ0sUZPBnEYDNTyntqVNUZ6C0B2XqV7ZZmcdYTeRdERYDx57P_z-s53KEi93RmQZhd2FldXMY9dIVW7kUv778ADJ0IDw priority: 102 providerName: ProQuest |
| Title | LC-MS Profiling of Kakkonto and Identification of Ephedrine as a Key Component for Its Anti-Glycation Activity |
| URI | https://cir.nii.ac.jp/crid/1870302168130690048 https://www.ncbi.nlm.nih.gov/pubmed/37298887 https://www.proquest.com/docview/2824005660 https://www.proquest.com/docview/2824693763 https://pubmed.ncbi.nlm.nih.gov/PMC10254259 https://doaj.org/article/60126867ef1243a1865d2859fba785f8 |
| Volume | 28 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3Nb9MwFH-CcYAL4puMrTISJ6Roses4zrGr2g3GpgmY1FvkT60apNPaHfbf816cVisgceGSQ2xLjn_P7yPP_j2AD4UPpZNK5bYqUwmz3Lrg8sJFU_toEHbK6J6eqeML-XlWzu6V-qIzYYkeOC3cAQYMQmlVhYiWaGi4VqUn0rVoTaXL2F3zRZu3Dqb6UIujXUo5zCEG9Qc_U6nZsBSaUzaz3rJCHVk_2pZ2Pv-bn_n7ccl79mf6DJ72jiMbpQk_hwehfQGPx-t6bS-h_TLOT7-x864INxoktojsxFxdEUEBM61n6U5u7H_SUfPk-jJ4uv7HzJIZdhLuGOmHRYsdGXqz7NNqyUY4KD_6cdcPG7lUb-IVXEwn38fHeV9NIXel5Ks8Su402iYpq6B5qHy0RShNicsZa8vr2muL3kKoYumVtbi3rTdm6AqDcBqphq9hp8UJvAXmfCyICE8QzkIUGscLJeoCUY9Cugw-rle3uU6kGQ0GGwRF8wcUGRzS-m86Et919wKloOmloPmXFGSwj-g1bk5PrkmNCa40GmiM_lFJZbC3xrXpN-mywWhTEhWqKjJ4v2lG1ChnYtqwuE19VE1aOIM3SQw2M6WMp0YlnYHeEpCtT9luaeeXHYU3JxICjDx3_8fHv4MnAl0vOtMg6j3YWd3chn10lVZ2AA-rWYVPPT0awKPDydn510G3U34BEQoSwg |
| linkProvider | Directory of Open Access Journals |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6VcigXxLsuLSwSXJCs2ht7vT4gFELThCQVEq3Um1nvg0YFO61Tof4pfiMzfgQCiFsvPnh3vY8Zz2Nndz6Al4GxsY6E8PMkbiDM_Fxb7QfaqdQ4hWSniO7sSIxOog-n8ekG_OjuwtCxyk4m1oLalJr2yPfRNYgob6UI3i4ufEKNouhqB6HRsMXEXn9Hl616M36P9H3F-fDgeDDyW1QBX8dRuPRdFGqJMjqKEitDmxiXBzZWcWy4S_MwTY3MUWvaxMVG5Dl2mhulejpQOC0ViR5-9xbcjnqoyelm-vBw5eCFqA2byCkWBvvfGoBbW3EZUgw1XdN9NUQAarRiPv-XdfvnIc3ftN7wHtxtzVXWb_jrPmzY4gFsDTqUuIdQTAf-7BP7WEN_oxpkpWMTdX5OaRGYKgxrbgK7dmuQig8WZ9bQpUOmKqYYLicjqVQWWJGhDc3Gy4r1sZF_-PW6bdbXDcrFIzi5kdV-DJsFDmAbmDYuoPR7nLiL80Biey54GiCvOR5pD153q5stmlQdGbo4RIrsL1J48I7Wf1WRsmzXL8rLL1n702borHKBPVqHVlBPYec4bBmnLleJjJ30YA-pl-k5PUNJwpOHQqJZIFISjR7sdnTNWtFQZb8Y2YMXq2KkGkVqVGHLq6aOSEn2e_CkYYPVSCnOKlE1eCDXGGRtKuslxfysThweUuoD9Hd3_j-u57A1Op5Ns-n4aPIU7nA06-i8BE93YXN5eWX30Axb5s9q3mfw-aZ_tp_SvkYt |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwED-NTgJeEN8ENjASvCBFTdzEsR8Q6rqWlW5VBUzaW3D8wapBWtZOaP8afx13TVIoIN72kofYTi6-833k7PsBvIisS00iRFhkaQVhFhbGmTAyXivrNbKdMrpHY3FwnLw7SU-24EdzFoa2VTY6caWo7czQP_I2hgYJ1a0UUdvX2yIm-4M3828hIUhRprWB06hEZOQuv2P4tng93Edev-R80P_YOwhrhIHQpEm8DH0SG4n6OkkyJ2OXWV9ELtVparlXRayUlQVaUJf51IqiQAIKq3XHRBo_USeig8-9BtsZRUUt2N7rjyfv1-FejLaxyqN2Oipqf63gbt2Cy5gyqmrDEq4AA9C-ldPpv3zdP7ds_mYDB7fhVu28sm4lbXdgy5V34UavwYy7B-VhLzz6wCYrIHA0imzm2UifnVGRBKZLy6pzwb7-UUjN_fmps3QEkekF0wwnlJGOmpXYkaFHzYbLBevioPDtl8t6WNdUmBf34fhK5vsBtEok4BEwY31Exfg4yRrnkcTxXHAVoeR5npgAXjWzm8-rwh05BjzEivwvVgSwR_O_7kg1t1c3Zuef83oJ5xi6coFvdB59oo7GlyPZMlW-0JlMvQxgF7mXmyldY0mqlMdCopMgFCnKAHYavua1oljkv8Q6gOfrZuQa5W106WYXVR-hyBIE8LASgzWllHWVaCgCkBsCsvEpmy3l9HRVRjymQggY_T7-P13P4DoutPxwOB49gZscfTzaPMHVDrSW5xduF32yZfG0Fn4Gn656vf0EXP5Lvw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=LC-MS+Profiling+of+Kakkonto+and+Identification+of+Ephedrine+as+a+Key+Component+for+Its+Anti-Glycation+Activity&rft.jtitle=Molecules&rft.au=Kaori+Ito&rft.au=Takashi+Kikuchi&rft.au=Kanako+Ikube&rft.au=Kouharu+Otsuki&rft.date=2023-05-29&rft.pub=MDPI+AG&rft.eissn=1420-3049&rft.volume=28&rft.spage=4409&rft_id=info:doi/10.3390%2Fmolecules28114409 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1420-3049&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1420-3049&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1420-3049&client=summon |