TERRA-LSD1 phase separation promotes R-loop formation for telomere maintenance in ALT cancer cells

• Published in: Nature communications Vol. 15; no. 1; pp. 2165 - 19
• Main Authors: Xu, Meng, Senanayaka, Dulmi, Zhao, Rongwei, Chigumira, Tafadzwa, Tripathi, Astha, Tones, Jason, Lackner, Rachel M., Wondisford, Anne R., Moneysmith, Laurel N., Hirschi, Alexander, Craig, Sara, Alishiri, Sahar, O’Sullivan, Roderick J., Chenoweth, David M., Reiter, Nicholas J., Zhang, Huaiying
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.03.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 14/1; 14/32; 14/34; 14/35; 14/63; 631/57/2272/2276; 631/80/103/560; 631/80/304; 82/83; Binding; Chromatin; Deoxyribonucleic acid; DNA; DNA biosynthesis; Histones; Homology; Humanities and Social Sciences; Lysine; Maintenance; multidisciplinary; Phase separation; R-loops; Ribonucleic acid; RNA; RNA modification; Science; Science (multidisciplinary); Synthesis; Telomerase; Telomeres; Yeast

The telomere repeat-containing RNA (TERRA) forms R-loops to promote homology-directed DNA synthesis in the alternative lengthening of telomere (ALT) pathway. Here we report that TERRA contributes to ALT via interacting with the lysine-specific demethylase 1A (LSD1 or KDM1A). We show that LSD1 locali...