Control of cyclic oligoadenylate synthesis in a type III CRISPR system

• Published in: eLife Vol. 7
• Main Authors: Rouillon, Christophe, Athukoralage, Januka S, Graham, Shirley, Grüschow, Sabine, White, Malcolm F
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 02.07.2018; eLife Sciences Publications, Ltd
• Subjects: Adaptive immunity; Adenine Nucleotides - biosynthesis; Antiviral drugs; archaea; Biochemistry and Chemical Biology; Cas10; Chromatography; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; CRISPR-Cas Systems; cyclic oligoadenylate; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Endodeoxyribonucleases - genetics; Endodeoxyribonucleases - metabolism; Endoribonucleases - genetics; Endoribonucleases - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Gene expression; Genomes; Kinetics; Mass spectrometry; Nuclease; Oligoribonucleotides - biosynthesis; Phosphorothioate; Phosphorothioate Oligonucleotides - pharmacology; Prokaryotes; Proteins; Ribonucleic acid; RNA; RNA Cleavage; RNA modification; RNA viruses; RNA Viruses - genetics; RNA Viruses - metabolism; RNA, Viral - genetics; RNA, Viral - metabolism; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Scientific imaging; Sulfolobus solfataricus; Sulfolobus solfataricus - drug effects; Sulfolobus solfataricus - genetics; Sulfolobus solfataricus - immunology; Sulfolobus solfataricus - metabolism; Time Factors; Transcription factors; Viral infections; CRISPR; chemical biology; biochemistry; Cas10; Sulfolobus solfataricus; cyclic oligoadenylate; archaea

The CRISPR system for prokaryotic adaptive immunity provides RNA-mediated protection from viruses and mobile genetic elements. When viral RNA transcripts are detected, type III systems adopt an activated state that licenses DNA interference and synthesis of cyclic oligoadenylate (cOA). cOA activates...