Evaluation and validation of suitable reference genes for quantitative real-time PCR analysis in lotus (Nelumbo nucifera Gaertn.)

• Published in: Scientific reports Vol. 14; no. 1; pp. 10857 - 9
• Main Authors: Wang, Bin, Zhu, Fenglin, Zheng, Xingwen, Yang, Liangbo, Diao, Ying, Hu, Zhongli
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.05.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647; 631/337; 631/449; Aquatic plants; Cultural values; Developmental stages; Gene expression; Gene Expression Profiling - methods; Gene Expression Profiling - standards; Gene Expression Regulation, Plant; Genes, Plant; geNorm; Glyceraldehyde-3-phosphate dehydrogenase; Humanities and Social Sciences; Lotus; Lotus - genetics; Lotus - growth & development; multidisciplinary; Nelumbo - genetics; Nelumbo nucifera; NormFinder; qRT-PCR; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - standards; Reference gene; Reference Standards; Science; Science (multidisciplinary); NormFinder; Reference gene; Lotus; Gene expression; geNorm; qRT-PCR

The qRT-PCR technique has been regarded as an important tool for assessing gene expression diversity. Selection of appropriate reference genes is essential for validating deviation and obtaining reliable and accurate results. Lotus ( Nelumbo nucifera Gaertn) is a common aquatic plant with important...