Ambra1 spatially regulates Src activity and Src/FAK-mediated cancer cell invasion via trafficking networks

• Published in: eLife Vol. 6
• Main Authors: Schoenherr, Christina, Byron, Adam, Sandilands, Emma, Paliashvili, Ketevan, Baillie, George S, Garcia-Munoz, Amaya, Valacca, Cristina, Cecconi, Francesco, Serrels, Bryan, Frame, Margaret C
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 31.03.2017; eLife Sciences Publications, Ltd
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Ambra1; Animals; Autophagy; Cancer; Cancer Biology; Carcinoma, Squamous Cell - physiopathology; Cell Adhesion; Cell adhesion & migration; Cell Biology; Cell Line, Tumor; Cell Movement; Dynactin; Dynactin Complex - metabolism; FAK; Focal adhesion kinase; Focal Adhesion Kinase 1 - metabolism; IFITM3; invasion; Invasiveness; Kinases; Medical research; Membrane Proteins - metabolism; Mice; Neurogenesis; Phagocytosis; Proteins; Proteomics; Squamous cell carcinoma; Src; Src protein; src-Family Kinases - metabolism; trafficking; IFITM3; FAK; mouse; cell biology; invasion; Ambra1; Src; cancer biology; trafficking

Here, using mouse squamous cell carcinoma cells, we report a completely new function for the autophagy protein Ambra1 as the first described 'spatial rheostat' controlling the Src/FAK pathway. Ambra1 regulates the targeting of active phospho-Src away from focal adhesions into autophagic st...