A gene-specific T2A-GAL4 library for Drosophila

• Published in: eLife Vol. 7
• Main Authors: Lee, Pei-Tseng, Zirin, Jonathan, Kanca, Oguz, Lin, Wen-Wen, Schulze, Karen L, Li-Kroeger, David, Tao, Rong, Devereaux, Colby, Hu, Yanhui, Chung, Verena, Fang, Ying, He, Yuchun, Pan, Hongling, Ge, Ming, Zuo, Zhongyuan, Housden, Benjamin E, Mohr, Stephanie E, Yamamoto, Shinya, Levis, Robert W, Spradling, Allan C, Perrimon, Norbert, Bellen, Hugo J
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 22.03.2018; eLife Sciences Publications, Ltd
• Subjects: cassette excision; Chromosomes and Gene Expression; complementation; CRIMIC; CRISPR; Deoxyribonucleic acid; DNA; Drosophila; GAL4/UAS; Gene expression; Genetic engineering; Immunoglobulins; Insects; Localization; loss of function; Medicine; MiMIC; Mutation; Phenotypes; Polyadenylation; Proteins; Tools and Resources; Transcription; United States > US; cassette excision; GAL4/UAS; MiMIC; CRIMIC; chromosomes; D. melanogaster; loss of function; complementation; gene expression

We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3’ of the GAL4. This allows numerous applications. First, ~90% of in...