Digital nanoreactors to control absolute stoichiometry and spatiotemporal behavior of DNA receptors within lipid bilayers

• Published in: Nature communications Vol. 14; no. 1; p. 1532
• Main Authors: Maingi, Vishal, Zhang, Zhao, Thachuk, Chris, Sarraf, Namita, Chapman, Edwin R., Rothemund, Paul W. K.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.03.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 147/143; 631/92/552; 639/638/92/314; 639/925/926/1050; 639/925/927; Carrier Proteins; Cell survival; Deoxyribonucleic acid; DNA; Humanities and Social Sciences; Kinetics; Lipid bilayers; Lipid Bilayers - metabolism; Lipids; Liposomes; Logic circuits; Membrane proteins; Membrane Proteins - metabolism; Membranes; multidisciplinary; Nanotechnology; Protein interaction; Proteins; Receptors; Science; Science (multidisciplinary); Signaling; Stoichiometry; Tethering

Interactions between membrane proteins are essential for cell survival but are often poorly understood. Even the biologically functional ratio of components within a multi-subunit membrane complex—the native stoichiometry—is difficult to establish. Here we demonstrate digital nanoreactors that can c...