Affinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a

• Published in: Nature communications Vol. 15; no. 1; pp. 9304 - 16
• Main Authors: Shen, Shimei, Wang, Wen, Ma, Yuanyan, Wang, Shilei, Zhang, Shaocheng, Cai, Xuefei, Chen, Liang, Zhang, Jin, Li, Yalan, Wu, Xiaoli, Wei, Jie, Zhao, Yanan, Huang, Ailong, Niu, Siqiang, Wang, Deqiang
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.10.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 59; 631/208/4041; 631/61/32; 692/53/2421; 692/699/255/1672; Affinity; Bacterial Proteins; Bronchoalveolar Lavage Fluid - microbiology; Bronchus; Candida albicans - genetics; Candida albicans - isolation & purification; Candidiasis - diagnosis; Candidiasis - microbiology; Chitin; Chitin - metabolism; CRISPR; CRISPR-Associated Proteins; CRISPR-Cas Systems; Diagnostic systems; DNA, Fungal - genetics; Drug resistance; Endodeoxyribonucleases - genetics; Endodeoxyribonucleases - metabolism; Fungi; Humanities and Social Sciences; Humans; Lavage; Magnetic separation; Morbidity; multidisciplinary; Science; Science (multidisciplinary); Sensitivity; Sensitivity and Specificity

Invasive fungal infections (IFIs) pose a significant threat to immunocompromised individuals, leading to considerable morbidity and mortality. Prompt and accurate diagnosis is essential for effective treatment. Here we develop a rapid molecular diagnostic method that involves three steps: fungal enr...