The Reversible Modification Regulates the Membrane-Binding State of Apg8/Aut7 Essential for Autophagy and the Cytoplasm to Vacuole Targeting Pathway

• Published in: The Journal of cell biology Vol. 151; no. 2; pp. 263 - 275
• Main Authors: Kirisako, Takayoshi, Ichimura, Yoshinobu, Okada, Hisashi, Kabeya, Yukiko, Mizushima, Noboru, Yoshimori, Tamotsu, Ohsumi, Mariko, Takao, Toshifumi, Noda, Takeshi, Ohsumi, Yoshinori
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 16.10.2000; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Anatomy & physiology; Antibodies; Autophagy; Autophagy-Related Protein 8 Family; Autophagy-Related Proteins; Biological Transport; Catalytic Domain; Cells; Cysteine Endopeptidases - metabolism; Cytoplasm; Cytoplasm - metabolism; Enzymes; Immunoblotting; Ligases - metabolism; Membrane Proteins - metabolism; Membranes; Microtubule-Associated Proteins - metabolism; Models, Biological; Molecular Sequence Data; Molecules; Original; Peroxisomes - metabolism; Plasmids; Protein Binding; Protein Processing, Post-Translational; Protein Sorting Signals; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Ubiquitin-Protein Ligases; Ubiquitins; Ubiquitins - metabolism; Vacuoles; Vacuoles - metabolism; Yeasts

Autophagy and the Cvt pathway are examples of nonclassical vesicular transport from the cytoplasm to the vacuole via double-membrane vesicles. Apg8/Aut7, which plays an important role in the formation of such vesicles, tends to bind to membranes in spite of its hydrophilic nature. We show here that...