The antibacterial activity of clove oil/chitosan nanoparticles embedded gelatin nanofibers against Escherichia coli O157:H7 biofilms on cucumber

This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO)...

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Published inInternational journal of food microbiology Vol. 266; pp. 69 - 78
Main Authors Cui, Haiying, Bai, Mei, Rashed, Marwan M.A., Lin, Lin
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 02.02.2018
Elsevier BV
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Abstract This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9mg/mL gelatin/CO@CNPs treatment for 24h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6mg/mL and 9mg/mL gelatin/CO@CNPs nanofibers treatment at 12°C for 4days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days. •The clove oil-loaded chitosan nanoparticles (CO@CNPs) were prepared.•The gelatin nanofibers containing CO@CNPs was prepared by electrospinning.•The gelatin nanofibers can eliminate E. coli O157:H7 biofilms.•The gelatin nanofibers can maintain sensory quality of cucumber.
AbstractList This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9mg/mL gelatin/CO@CNPs treatment for 24h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6mg/mL and 9mg/mL gelatin/CO@CNPs nanofibers treatment at 12°C for 4days, 4.28 and 4.97 log reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days.
This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9mg/mL gelatin/CO@CNPs treatment for 24h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6mg/mL and 9mg/mL gelatin/CO@CNPs nanofibers treatment at 12°C for 4days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days.
This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9mg/mL gelatin/CO@CNPs treatment for 24h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6mg/mL and 9mg/mL gelatin/CO@CNPs nanofibers treatment at 12°C for 4days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days. •The clove oil-loaded chitosan nanoparticles (CO@CNPs) were prepared.•The gelatin nanofibers containing CO@CNPs was prepared by electrospinning.•The gelatin nanofibers can eliminate E. coli O157:H7 biofilms.•The gelatin nanofibers can maintain sensory quality of cucumber.
This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9mg/mL gelatin/CO@CNPs treatment for 24h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6mg/mL and 9mg/mL gelatin/CO@CNPs nanofibers treatment at 12°C for 4days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days.This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9mg/mL gelatin/CO@CNPs treatment for 24h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6mg/mL and 9mg/mL gelatin/CO@CNPs nanofibers treatment at 12°C for 4days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for >4days.
This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of clove oil (CO) was 2.5 mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8 h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9 mg/mL gelatin/CO@CNPs treatment for 24 h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6 mg/mL and 9 mg/mL gelatin/CO@CNPs nanofibers treatment at 12 °C for 4 days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for > 4 days.
Author Bai, Mei
Rashed, Marwan M.A.
Lin, Lin
Cui, Haiying
Author_xml – sequence: 1
  givenname: Haiying
  surname: Cui
  fullname: Cui, Haiying
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  givenname: Mei
  surname: Bai
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  givenname: Marwan M.A.
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  fullname: Rashed, Marwan M.A.
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  givenname: Lin
  orcidid: 0000-0001-6374-2165
  surname: Lin
  fullname: Lin, Lin
  email: linl@ujs.edu.cn
BackLink https://www.ncbi.nlm.nih.gov/pubmed/29179098$$D View this record in MEDLINE/PubMed
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ISSN 0168-1605
1879-3460
IngestDate Tue Aug 05 10:58:43 EDT 2025
Fri Jul 11 07:53:17 EDT 2025
Wed Aug 13 04:39:57 EDT 2025
Wed Feb 19 02:43:10 EST 2025
Thu Apr 24 23:08:00 EDT 2025
Tue Jul 01 01:58:05 EDT 2025
Fri Feb 23 02:29:01 EST 2024
IsPeerReviewed true
IsScholarly true
Keywords Clove oil
Chitosan nanoparticles
Biofilms
Nanofibers
Cucumber
Escherichia coli O157:H7
Language English
License Copyright © 2017 Elsevier B.V. All rights reserved.
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Snippet This study aims to evaluate the antibacterial activity of clove oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against...
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SubjectTerms Antibacterial activity
antibacterial properties
Bacteria
biofilm
Biofilms
Chitosan
Chitosan nanoparticles
Clove oil
cloves
color
Crosslinking
Cucumber
Cucumbers
E coli
Escherichia coli
Escherichia coli O157
Escherichia coli O157:H7
Essential oils
flavor
Flavors
Gelatin
Nanofibers
Nanoparticles
Sensory evaluation
Vegetables
Title The antibacterial activity of clove oil/chitosan nanoparticles embedded gelatin nanofibers against Escherichia coli O157:H7 biofilms on cucumber
URI https://dx.doi.org/10.1016/j.ijfoodmicro.2017.11.019
https://www.ncbi.nlm.nih.gov/pubmed/29179098
https://www.proquest.com/docview/2017034712
https://www.proquest.com/docview/1969924377
https://www.proquest.com/docview/2053892602
Volume 266
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