Development of RAPD-PCR assay for identifying Holstein, Angus, and Taiwan Yellow Cattle for meat adulteration detection

• Published in: Food science and biotechnology Vol. 28; no. 6; pp. 1769 - 1777
• Main Authors: Lin, Chin-Cheng, Tang, Pin-Chi, Chiang, Hsin-I
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.12.2019; Springer Nature B.V; 한국식품과학회
• Subjects: adulterated products; Amplification; Angus; beef; Beef cattle; biotechnology; Cattle; Chemistry; Chemistry and Materials Science; DNA; DNA probes; food fraud; Food Science; Fraud; Holstein; Meat; Nucleotides; Nutrition; Polymerase chain reaction; Random amplified polymorphic DNA; random amplified polymorphic DNA technique; Reproducibility; Taiwan; 식품과학; Taiwan; Food adulteration; RAPD-PCR; Meat product; Cattle; Species identification

Incidents of food fraud have occurred worldwide, particularly in the form of meat adulteration. In this study, molecular probes were developed using the Random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique in order to identify three beef subspecies–Holstein, Angus...