Weighted gene co-expression network analysis reveals genes related to growth performance in Hu sheep

• Published in: Scientific reports Vol. 14; no. 1; pp. 13043 - 12
• Main Authors: Wang, Qiang, Xu, Jie, Bao, Menghuan, Wang, Huining, Sun, XiaoMei, Ji, Dejun, Wang, Jian, Li, Yongjun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.06.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114/2114; 631/114/2403; 631/114/2404; Animals; Body height; Body length; Body Weight - genetics; Breeding; Chest; Female; Gene Expression Profiling; Gene Regulatory Networks; Genes; Growth performance; Hu sheep; Humanities and Social Sciences; Livestock; Male; Meat; Meat quality; Metabolic pathways; multidisciplinary; RNA sequencing; Science; Science (multidisciplinary); Sheep; Sheep - genetics; Sheep - growth & development; Slaughter; Slaughter performance; Transcriptome; Transcriptomes; WGCNA; RNA sequencing; Slaughter performance; WGCNA; Hu sheep; Growth performance; Meat quality
• DOI: 10.1038/s41598-024-63850-x
• ISSN: 2045-2322

Hu sheep are a unique breed in our country with great reproductive potential, the extent of whose breeding has been steadily rising in recent years. The study subjects in this experiment were 8-month-old Hu sheep (n = 112). First of all, the growth performance, slaughter performance and meat quality of their eye muscle quality were assessed, meanwhile their live weight, carcass weight, body length, body height, chest circumference, chest depth and tube circumference were respectively 33.81 ± 5.47 kg, 17.43 ± 3.21 kg, 60.36 ± 4.41 cm, 63.25 ± 3.88 cm, 72.03 ± 5.02 cm, 30.70 ± 2.32 cm and 7.36 ± 0.56 cm, with a significant difference between rams and ewes ( P  < 0.01). Following that, transcriptome sequencing was done, and candidate genes related to growth performance were identified using the weighted co-expression network analysis (WGCNA) approach, which was used to identified 15 modules, with the turquoise and blue modules having the strongest association with growth and slaughter performance, respectively. We discovered hub genes such as ARHGAP31, EPS8, AKT3, EPN1, PACS2, KIF1C, C12H1orf115, FSTL1, PTGFRN and IFIH1 in the gene modules connected with growth and slaughter performance. Our research identifies the hub genes associated with the growth and slaughter performance of Hu sheep, which play an important role in their muscle growth, organ and cartilage development, blood vessel development and energy metabolic pathways. Our findings might lead to the development of potentially-useful biomarkers for the selection of growth and slaughterer performance-related attributes of sheep and other livestock.