Fanzor is a eukaryotic programmable RNA-guided endonuclease

• Published in: Nature (London) Vol. 620; no. 7974; pp. 660 - 668
• Main Authors: Saito, Makoto, Xu, Peiyu, Faure, Guilhem, Maguire, Samantha, Kannan, Soumya, Altae-Tran, Han, Vo, Sam, Desimone, AnAn, Macrae, Rhiannon K., Zhang, Feng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.08.2023; Nature Publishing Group
• Subjects: 42/44; 45/91; 631/45; 631/535; Adaptive immunity; Adaptive systems; Archaea; Archaea - genetics; Archaea - immunology; Bacteria; Bacteria - genetics; Bacteria - immunology; Biological activity; Chytridiomycota - enzymology; Complementarity; Conservation; Conserved Sequence; CRISPR; CRISPR-Associated Protein 9 - metabolism; CRISPR-Associated Proteins - chemistry; CRISPR-Associated Proteins - metabolism; CRISPR-Associated Proteins - ultrastructure; CRISPR-Cas Systems; Cryoelectron Microscopy; Deoxyribonucleic acid; DNA; DNA Transposable Elements - genetics; Electron microscopy; Endonuclease; Endonucleases - chemistry; Endonucleases - metabolism; Endonucleases - ultrastructure; Eukaryota - enzymology; Eukaryotes; Evolution, Molecular; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Fungal Proteins - ultrastructure; Fungi; Gene Editing - methods; Gene sequencing; Genes; Genome editing; Genomes; Humanities and Social Sciences; Humans; multidisciplinary; Nucleic acids; Prokaryotes; Proteins; Ribonucleic acid; RNA; RNA - genetics; RNA - metabolism; RNA modification; RNA, Guide, CRISPR-Cas Systems - genetics; RNA, Guide, CRISPR-Cas Systems - metabolism; RNA-mediated interference; rRNA; Science; Science (multidisciplinary)

RNA-guided systems, which use complementarity between a guide RNA and target nucleic acid sequences for recognition of genetic elements, have a central role in biological processes in both prokaryotes and eukaryotes. For example, the prokaryotic CRISPR–Cas systems provide adaptive immunity for bacte...