The Utilization of a Commercial Soil Nucleic Acid Extraction Kit and PCR for the Detection of Clostridium tetanus and Clostridium chauvoei on Farms after Flooding in Taiwan

• Published in: Journal of Veterinary Medical Science Vol. 75; no. 4; pp. 489 - 495
• Main Authors: HUANG, Shr-Wei, CHAN, Jacky Peng-Wen, SHIA, Wei-Yau, SHYU, Chin-Lin, TUNG, Kwon-Chung, WANG, Chi-Young
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2013; Japan Science and Technology Agency
• Subjects: Animals; bacterial zoonoses; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Clostridium chauvoei - genetics; Clostridium chauvoei - isolation & purification; Clostridium Infections - epidemiology; Clostridium tetani - genetics; Clostridium tetani - isolation & purification; diagnose; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Floods; PCR; Polymerase Chain Reaction - veterinary; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; Soil Microbiology; Taiwan - epidemiology; Zoonoses - epidemiology; Zoonoses - microbiology; Taiwan

Clostridial diseases are zoonoses and are classified as soil-borne diseases. Clostridium chauvoei and Clostridium tetani cause blackleg disease and tetanus, respectively. Since bacteria and spores are re-distributed by floods and then, subsequently, contaminate soils, pastures and water; the case nu...