Illuminating the dark space of neutral glycosphingolipidome by selective enrichment and profiling at multi-structural levels

• Published in: Nature communications Vol. 15; no. 1; pp. 5627 - 11
• Main Authors: Wang, Zidan, Zhang, Donghui, Wu, Junhan, Zhang, Wenpeng, Xia, Yu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.07.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 140/58; 631/45/608; 631/61/320; 639/638/11/296; Animals; Brain; Brain - metabolism; Brain mapping; Brain Neoplasms - genetics; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Cell membranes; Chromatography, Liquid - methods; Desaturation; Enrichment; Gene mapping; Glioma; Glioma - genetics; Glioma - metabolism; Glioma - pathology; Glycosphingolipids; Glycosphingolipids - chemistry; Glycosphingolipids - metabolism; Harnesses; Humanities and Social Sciences; Humans; Hydroxylation; Isocitrate dehydrogenase; Isocitrate Dehydrogenase - genetics; Isocitrate Dehydrogenase - metabolism; Isomers; Lipidomics - methods; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Mice; multidisciplinary; Nanoparticles; Nervous system; Peptide mapping; Phospholipids; Science; Science (multidisciplinary); Tandem Mass Spectrometry - methods; Titanium dioxide; Workflow

Glycosphingolipids (GSLs) are essential components of cell membranes, particularly enriched in the nervous system. Altered molecular distributions of GSLs are increasingly associated with human diseases, emphasizing the significance of lipidomic profiling. Traditional GSL analysis methods are hamper...