Transparent tissue in solid state for solvent-free and antifade 3D imaging

• Published in: Nature communications Vol. 14; no. 1; pp. 3395 - 12
• Main Authors: Hsiao, Fu-Ting, Chien, Hung-Jen, Chou, Ya-Hsien, Peng, Shih-Jung, Chung, Mei-Hsin, Huang, Tzu-Hui, Lo, Li-Wen, Shen, Chia-Ning, Chang, Hsiu-Pi, Lee, Chih-Yuan, Chen, Chien-Chia, Jeng, Yung-Ming, Tien, Yu-Wen, Tang, Shiue-Cheng
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.06.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/51; 14/19; 14/34; 631/1647/245/2225; 631/1647/328/2238; 631/61/54/990; 692/698/1688/1315/1951; 692/698/2741/416; Acrylamide; Animals; Clearing; Copolymers; Dyes; Evaporation; Fluorescent Dyes; Fluorescent indicators; Human tissues; Humanities and Social Sciences; Humans; Image resolution; Imaging, Three-Dimensional - methods; Laboratory tests; Mice; multidisciplinary; Optical Imaging; Photobleaching; Photochemical reactions; Polymers; Reagents; Science; Science (multidisciplinary); Solid state; Solvents; Three dimensional imaging; Tissues

Optical clearing with high-refractive-index (high- n ) reagents is essential for 3D tissue imaging. However, the current liquid-based clearing condition and dye environment suffer from solvent evaporation and photobleaching, causing difficulties in maintaining the tissue optical and fluorescent feat...