B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk

• Published in: eLife Vol. 3; p. e02069
• Main Authors: Kläsener, Kathrin, Maity, Palash C, Hobeika, Elias, Yang, Jianying, Reth, Michael
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 24.06.2014; eLife Sciences Publications, Ltd
• Subjects: Animals; Annealing; Antigens; B cell antigen receptor; B-cell receptor; B-Lymphocytes - metabolism; CD19 antigen; Cell activation; Cell Line; Conformation; Cytoskeleton; Deoxyribonucleic acid; Dimerization; DNA; Exports; Flow cytometry; Humans; Immunoglobulin D; Immunoglobulin M; Immunoglobulins; Immunology; inside-out signaling; Lymphocyte Activation; Lymphocytes B; Mice; nanoscale receptor organization; Plasmids; Proteins; Receptor density; Receptors, Antigen, B-Cell - metabolism; Signal Transduction; Software; Syk protein; nanoscale receptor organization; B cell antigen receptor; inside-out signaling

Binding of antigen to the B cell antigen receptor (BCR) initiates a multitude of events resulting in B cell activation. How the BCR becomes signaling-competent upon antigen binding is still a matter of controversy. Using a high-resolution proximity ligation assay (PLA) to monitor the conformation of...