Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides

• Published in: Nature communications Vol. 15; no. 1; pp. 4336 - 13
• Main Authors: Liu, Wan-Qiu, Ji, Xiangyang, Ba, Fang, Zhang, Yufei, Xu, Huiling, Huang, Shuhui, Zheng, Xiao, Liu, Yifan, Ling, Shengjie, Jewett, Michael C., Li, Jian
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.05.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/92/60/1167; 639/638/92/349/977; 639/638/92/60/1167; 82/80; Antimicrobial activity; Biocatalysis; Biological activity; Biosynthesis; Biosynthetic Pathways - genetics; Catalysis; Cell-Free System; Clusters; Gene clusters; Gene expression; Genomes; Humanities and Social Sciences; Microorganisms; multidisciplinary; Multigene Family; Natural products; Peptides; Peptides - chemistry; Peptides - genetics; Peptides - metabolism; Post-translation; Protein Processing, Post-Translational; Ribosomes - genetics; Ribosomes - metabolism; Science; Science (multidisciplinary)

Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a major class of natural products with diverse chemical structures and potent biological activities. A vast majority of RiPP gene clusters remain unexplored in microbial genomes, which is partially due to the lack of rapi...