The AAV capsid can influence the epigenetic marking of rAAV delivered episomal genomes in a species dependent manner

• Published in: Nature communications Vol. 14; no. 1; pp. 2448 - 11
• Main Authors: Gonzalez-Sandoval, Adriana, Pekrun, Katja, Tsuji, Shinnosuke, Zhang, Feijie, Hung, King L., Chang, Howard Y., Kay, Mark A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.04.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/1; 13/106; 13/107; 13/109; 38; 38/44; 42; 42/44; 45; 631/208/176; 631/326/596/2561; 631/45/612/100/2285; 631/61/201; 631/61/2300/1514; Amino acids; Animals; Capsid - metabolism; Capsid protein; Capsid Proteins - genetics; Capsid Proteins - metabolism; Capsids; Chromatin; Chromatin - genetics; Chromatin - metabolism; Deoxyribonucleic acid; Dependovirus - metabolism; Discordance; DNA; Epigenesis, Genetic; Epigenetics; Gene therapy; Genetic Vectors - genetics; Genomes; Histone H3; Histones; Humanities and Social Sciences; Humans; Mice; multidisciplinary; Nuclear transport; Proteins; Science; Science (multidisciplinary); Species; Transduction; Transduction, Genetic; Uncoating; Vectors

Recombinant adeno-associated viral vectors (rAAVs) are among the most commonly used vehicles for in vivo based gene therapies. However, it is hard to predict which AAV capsid will provide the most robust expression in human subjects due to the observed discordance in vector-mediated transduction bet...