Deep-profiling of phospholipidome via rapid orthogonal separations and isomer-resolved mass spectrometry

• Published in: Nature communications Vol. 14; no. 1; pp. 4263 - 12
• Main Authors: Xia, Tian, Zhou, Feng, Zhang, Donghui, Jin, Xue, Shi, Hengxue, Yin, Hang, Gong, Yanqing, Xia, Yu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.07.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 140/58; 631/45/320; 639/638/11/296; Animals; Bladder cancer; Cattle; Chromatography; Chromatography, Liquid; Data analysis; Depth profiling; Desaturation; Glycerophospholipids - analysis; Humanities and Social Sciences; Humans; Hydrophilicity; Ionic mobility; Ions; Isobars; Isomers; Lecithin; Lipid metabolism; Lipids; Lipids - analysis; Liquid chromatography; Liver; Macrophages; Mass spectrometry; Mass spectroscopy; Mice; Mobility; multidisciplinary; Phenotyping; Phosphatidylcholine; Phosphatidylcholines; Phospholipids; Phospholipids - analysis; RAW 264.7 Cells; Science; Science (multidisciplinary); Scientific imaging; Software; Sphingomyelins - analysis; Tandem Mass Spectrometry; Urinary Bladder Neoplasms

A lipidome comprises thousands of lipid species, many of which are isomers and isobars. Liquid chromatography-tandem mass spectrometry (LC-MS/MS), although widely used for lipidomic profiling, faces challenges in differentiating lipid isomers. Herein, we address this issue by leveraging the orthogon...