A Lipopolysaccharide Synthesis Gene rfaD from Mesorhizobium huakuii Is Involved in Nodule Development and Symbiotic Nitrogen Fixation

• Published in: Microorganisms (Basel) Vol. 11; no. 1; p. 59
• Main Authors: Liu, Yuan, Lin, Ye, Guan, Ning, Song, Yuting, Li, Youguo, Xie, Xianan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.12.2022; MDPI
• Subjects: Antigens; Astragalus sinicus; Bacteria; Biosynthesis; Cell division; Cell walls; Deletion; Deletion mutant; E coli; Gene expression; genes; Glycerol; Gram-negative bacteria; Growth rate; Host plants; Infections; Legumes; Lipids; lipopolysaccharide; Lipopolysaccharides; Mesorhizobium huakuii; Mesorhizobium huakuii 7653R; Molecular modelling; Mutants; Nitrogen; Nitrogen fixation; Nitrogenation; Nodulation; Nodules; Permeability; phenotype; Phenotypes; rfaD; RfaD gene; Rhizobium; Root nodules; Seeds; Symbiosis; symbiotic nitrogen fixation; Synthesis; symbiotic nitrogen fixation; gram-negative bacteria; rfaD; lipopolysaccharide; Mesorhizobium huakuii 7653R

Rhizobium lipopolysaccharide (LPS) is an important component of the cell wall of gram-negative bacteria and serves as a signal molecule on the surface of rhizobia, participating in the symbiosis during rhizobia–legume interaction. In this study, we constructed a deletion mutant of ADP-L-glycerol-D-m...