Establishment of Five Canine Lymphoma Cell Lines and Tumor Formation in a Xenotransplantation Model

• Published in: Journal of Veterinary Medical Science Vol. 75; no. 4; pp. 467 - 474
• Main Authors: YAMAZAKI, Jumpei, OKUDA, Masaru, KUBO, Masahito, HAYASHI, Toshiharu, TSUJIMOTO, Hajime, SUZUKI, Ryoichi, EMA, Yasuo, OKAMURA, Yasuhiko, TANI, Kenji, HIRAOKA, Hiroko, UMEKI, Saori, MIZUNO, Takuya
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2013; Japan Science and Technology Agency
• Subjects: Animals; canine lymphoma; cell line; Cell Line, Tumor; Cell Proliferation; characterization; Dog Diseases - genetics; Dog Diseases - pathology; Dogs; Female; Immunophenotyping - veterinary; Lymphoma - genetics; Lymphoma - pathology; Lymphoma - veterinary; Male; Mice; Mice, Inbred NOD; Mice, SCID; Real-Time Polymerase Chain Reaction - veterinary; Receptors, Antigen, T-Cell - genetics; RNA, Neoplasm - chemistry; RNA, Neoplasm - genetics; Specific Pathogen-Free Organisms; Transplantation, Heterologous - veterinary; xenograft model

Five novel, canine lymphoma cell lines (Ema, CLC, CLK, Nody-1 and UL-1) were established from dogs suffering from lymphoma and characterized in vitro and in vivo. All cell lines, except CLC, were characterized with T-cell phenotypes, by flow cytometric analysis and polymerase chain reaction for anti...