Characterization of OP9 as authentic mesenchymal stem cell line

Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including osteocytes,chondrocytes,adipocytes,myocytes,and tenocytes.However,the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models....

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Published inJournal of genetics and genomics Vol. 37; no. 7; pp. 475 - 482
Main Authors Gao, Jiao, Yan, Xin-Long, Li, Ren, Liu, Yi, He, Wenyan, Sun, Shengkun, Zhang, Yu, Liu, Bing, Xiong, Jiaxiang, Mao, Ning
Format Journal Article
LanguageEnglish
Published China Elsevier Ltd 01.07.2010
Subjects
Animals
Bone Marrow Cells - cytology
Cell Differentiation - physiology
Cell Proliferation
Cells, Cultured
differentiation
Hematopoietic Stem Cells
immunoregulation
Insulin-Like Growth Factor I - immunology
Interleukin-3 - immunology
Lymphocyte Activation - physiology
Lymphocyte Culture Test, Mixed
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mice
mouse
Multipotent Stem Cells
OP9
Platelet-Derived Growth Factor - metabolism
Proto-Oncogene Proteins c-sis
Stem Cells - physiology
Stromal Cells - physiology
T-Lymphocytes
Transforming Growth Factor beta1 - immunology
Transforming Growth Factor beta3 - immunology
Transforming Growth Factor beta3 - physiology
淋巴细胞转化试验
细胞生物学特性
细胞系
表征
软骨细胞
间质干细胞
骨髓基质干细胞
骨髓基质细胞
mouse
OP9
immunoregulation
differentiation
mesenchymal stem cells
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Abstract Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including osteocytes,chondrocytes,adipocytes,myocytes,and tenocytes.However,the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models.The OP9,a stromal cell line from mouse bone marrow,has hematopoietic supportive capacity.Here,we report that the OP9 has the immunophenotype （CD45-,CD11b-,FLK-1-,CD31-,CD34-,CD44＋,CD29＋,Sca-1＋,CD86-,and MHCII-） identical to canonical mouse MSCs.The expression of CD140a＋,CD140b＋,α-SMA＋ and Calponin＋ suggested the perivascular origin of OP9.Functionally,the OP9 had strong clonogenic ability and could be induced into osteocytes,chondrocytes and adipocytes.The lymphocyte transformation test （LTT） and mixed leukocyte reaction （MLR） showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens （PHA） or allogeneic lymphocytes （BALB/c T cells）.Finally,the migration of OP9 could be efficiently induced by bFGF,IGF-1,IL-3,PDGF-BB,TGF-β1 and TGF-β3.In conclusion,the OP9 were bona fide MSCs,and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
AbstractList Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. However, the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models. The OP9, a stromal cell line from mouse bone marrow, has hematopoietic supportive capacity. Here, we report that the OP9 has the immunophenotype (CD45(-), CD11b(-), FLK-1(-), CD31(-), CD34(-), CD44(+), CD29(+), Sca-1(+), CD86(-), and MHCII(-)) identical to canonical mouse MSCs. The expression of CD140a(+), CD140b(+), alpha-SMA(+) and Calponin(+) suggested the perivascular origin of OP9. Functionally, the OP9 had strong clonogenic ability and could be induced into osteocytes, chondrocytes and adipocytes. The lymphocyte transformation test (LTT) and mixed leukocyte reaction (MLR) showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens (PHA) or allogeneic lymphocytes (BALB/c T cells). Finally, the migration of OP9 could be efficiently induced by bFGF, IGF-1, IL-3, PDGF-BB, TGF-beta1 and TGF-beta3. In conclusion, the OP9 were bona fide MSCs, and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including osteocytes,chondrocytes,adipocytes,myocytes,and tenocytes.However,the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models.The OP9,a stromal cell line from mouse bone marrow,has hematopoietic supportive capacity.Here,we report that the OP9 has the immunophenotype （CD45-,CD11b-,FLK-1-,CD31-,CD34-,CD44＋,CD29＋,Sca-1＋,CD86-,and MHCII-） identical to canonical mouse MSCs.The expression of CD140a＋,CD140b＋,α-SMA＋ and Calponin＋ suggested the perivascular origin of OP9.Functionally,the OP9 had strong clonogenic ability and could be induced into osteocytes,chondrocytes and adipocytes.The lymphocyte transformation test （LTT） and mixed leukocyte reaction （MLR） showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens （PHA） or allogeneic lymphocytes （BALB/c T cells）.Finally,the migration of OP9 could be efficiently induced by bFGF,IGF-1,IL-3,PDGF-BB,TGF-β1 and TGF-β3.In conclusion,the OP9 were bona fide MSCs,and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. However, the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models. The OP9, a stromal cell line from mouse bone marrow, has hematopoietic supportive capacity. Here, we report that the OP9 has the immunophenotype (CD45 −, CD11b −, FLK-1 −, CD31 −, CD34 −, CD44 +, CD29 +, Sca-1 +, CD86 −, and MHCII −) identical to canonical mouse MSCs. The expression of CD140a +, CD140b +, α-SMA + and Calponin + suggested the perivascular origin of OP9. Functionally, the OP9 had strong clonogenic ability and could be induced into osteocytes, chondrocytes and adipocytes. The lymphocyte transformation test (LTT) and mixed leukocyte reaction (MLR) showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens (PHA) or allogeneic lymphocytes (BALB/c T cells). Finally, the migration of OP9 could be efficiently induced by bFGF, IGF-1, IL-3, PDGF-BB, TGF-β1 and TGF-β3. In conclusion, the OP9 were bona fide MSCs, and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. However, the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models. The OP9, a stromal cell line from mouse bone marrow, has hematopoietic supportive capacity. Here, we report that the OP9 has the immunophenotype (CD45 super(-), CD11b super(-), FLK-1 super(-), CD31 super(-), CD34 super(-), CD44 super(+), CD29 super(+), Sca-1 super(+), CD86 super(-), and MHCII super(-)) identical to canonical mouse MSCs. The expression of CD140a super(+), CD140b super(+), a-SMA super(+) and Calponin super(+) suggested the perivascular origin of OP9. Functionally, the OP9 had strong clonogenic ability and could be induced into osteocytes, chondrocytes and adipocytes. The lymphocyte transformation test (LTT) and mixed leukocyte reaction (MLR) showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens (PHA) or allogeneic lymphocytes (BALB/c T cells). Finally, the migration of OP9 could be efficiently induced by bFGF, IGF-1, IL-3, PDGF-BB, TGF-b1 and TGF-b3. In conclusion, the OP9 were bona fide MSCs, and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
Author Jiao Gao Xin-Long Yan Ren Li Yi Liu Wenyan He Shengkun Sun Yu Zhang Bing Liu Jiaxiang Xiong Ning Mao 1
AuthorAffiliation Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China Department of Urology, PLA General Hospital, Beijing 100853, China Department of Immunology, Peking University Health Science Center, Beijing 100191, China Department of Physiology, Third Military Medical University, Chongqing 400038, China
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  surname: Gao
  fullname: Gao, Jiao
  organization: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China
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  surname: Yan
  fullname: Yan, Xin-Long
  organization: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China
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  organization: Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
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  organization: Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
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  organization: Department of Urology, PLA General Hospital, Beijing 100853, China
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  surname: Zhang
  fullname: Zhang, Yu
  organization: Department of Immunology, Peking University Health Science Center, Beijing 100191, China
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  surname: Liu
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  organization: Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
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  surname: Xiong
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  email: xiongjiaxiang@gmail.com
  organization: Department of Physiology, Third Military Medical University, Chongqing 400038, China
– sequence: 10
  givenname: Ning
  surname: Mao
  fullname: Mao, Ning
  email: maoning@nic.bmi.ac.cn
  organization: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China
BackLink https://www.ncbi.nlm.nih.gov/pubmed/20659712$$D View this record in MEDLINE/PubMed
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Keywords mouse
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immunoregulation
differentiation
mesenchymal stem cells
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Snippet Mesenchymal stem cells （MSCs） are multipotent stem cells capable of differentiating into various cell types,including...
Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes,...
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SubjectTerms Animals
Bone Marrow Cells - cytology
Cell Differentiation - physiology
Cell Proliferation
Cells, Cultured
differentiation
Hematopoietic Stem Cells
immunoregulation
Insulin-Like Growth Factor I - immunology
Interleukin-3 - immunology
Lymphocyte Activation - physiology
Lymphocyte Culture Test, Mixed
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mice
mouse
Multipotent Stem Cells
OP9
Platelet-Derived Growth Factor - metabolism
Proto-Oncogene Proteins c-sis
Stem Cells - physiology
Stromal Cells - physiology
T-Lymphocytes
Transforming Growth Factor beta1 - immunology
Transforming Growth Factor beta3 - immunology
Transforming Growth Factor beta3 - physiology
淋巴细胞转化试验
细胞生物学特性
细胞系
表征
软骨细胞
间质干细胞
骨髓基质干细胞
骨髓基质细胞
Title Characterization of OP9 as authentic mesenchymal stem cell line
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https://dx.doi.org/10.1016/S1673-8527(09)60067-9
https://www.ncbi.nlm.nih.gov/pubmed/20659712
https://search.proquest.com/docview/749024010
https://search.proquest.com/docview/754565815
Volume 37
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