Comprehensive analysis of disulfidptosis-related genes reveals the effect of disulfidptosis in ulcerative colitis

• Published in: Scientific reports Vol. 14; no. 1; pp. 15705 - 18
• Main Authors: Song, Huixian, Zhang, Fengrui, Bai, Xinyu, Liang, Hao, Niu, Junkun, Miao, Yinglei
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.07.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114/1305; 692/4020/1503/257/1389; 692/53/2421; Animals; Apoptosis; Apoptosis - genetics; Bioinformatics; Cell death; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - genetics; Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; Computational Biology - methods; Dextran; Dextran Sulfate; Disease Models, Animal; Disulfidptosis; Female; Gene expression; Gene Expression Profiling; Homeostasis; Humanities and Social Sciences; Humans; Immune infiltration; Infiltration; Inflammatory bowel disease; Inflammatory bowel diseases; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestine; Machine learning; Male; Metabolomics; Mice; Mice, Inbred C57BL; Mortality; mRNA; Mucosa; multidisciplinary; Pathogenesis; Science; Science (multidisciplinary); Ulcerative colitis; Weighted gene co-expression network analysis; Western blotting; Disulfidptosis; Machine learning; Immune infiltration; Ulcerative colitis; Weighted gene co-expression network analysis
• DOI: 10.1038/s41598-024-66533-9
• ISSN: 2045-2322

Ulcerative colitis (UC) is a chronic inflammatory condition of the intestinal tract. Various programmed cell death pathways in the intestinal mucosa are crucial to the pathogenesis of UC. Disulfidptosis, a recently identified form of programmed cell death, has not been extensively reported in the context of UC. This study evaluated the expression of disulfidptosis-related genes (DRGs) in UC through public databases and assessed disulfide accumulation in the intestinal mucosal tissues of UC patients and dextran sulfate sodium (DSS)-induced colitis mice via targeted metabolomics. We utilized various bioinformatics techniques to identify UC-specific disulfidptosis signature genes, analyze their potential functions, and investigate their association with immune cell infiltration in UC. The mRNA and protein expression levels of these signature genes were confirmed in the intestinal mucosa of DSS-induced colitis mice and UC patients. A total of 24 DRGs showed differential expression in UC. Our findings underscore the role of disulfide stress in UC. Four UC-related disulfidptosis signature genes—SLC7A11, LRPPRC, NDUFS1, and CD2AP—were identified. Their relationships with immune infiltration in UC were analyzed using CIBERSORT, and their expression levels were validated by quantitative real-time PCR and western blotting. This study provides further insights into their potential functions and explores their links to immune infiltration in UC. In summary, disulfidptosis, as a type of programmed cell death, may significantly influence the pathogenesis of UC by modulating the homeostasis of the intestinal mucosal barrier.