Design and application of a core genome multilocus sequence typing scheme for investigation of Legionnaires' disease incidents

• Published in: Euro surveillance : bulletin européen sur les maladies transmissibles Vol. 20; no. 28; p. 1
• Main Authors: Moran-Gilad, J, Prior, K, Yakunin, E, Harrison, T G, Underwood, A, Lazarovitch, T, Valinsky, L, Lück, C, Krux, F, Agmon, V, Grotto, I, Harmsen, D
• Format: Journal Article
• Language: English
• Published: Sweden Centre Europeen pour la Surveillance Epidemiologique du SIDA (European Centre for the Epidemiological Monitoring of AIDS) 16.07.2015
• Subjects: DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Genetic Variation; Genome, Bacterial; Genomes; Genomics; Humans; Legionella pneumophila - classification; Legionella pneumophila - genetics; Legionella pneumophila - isolation & purification; Legionnaires' disease; Molecular Epidemiology - methods; Molecular Sequence Data; Molecular Typing - methods; Multilocus Sequence Typing - methods; Pediatrics; Polymorphism; Polymorphism, Single Nucleotide; Sequence Analysis, DNA

Sequence-based typing (SBT) for Legionella pneumophila (Lp) has dramatically improved Legionnaires’ disease (LD) cluster investigation. Microbial whole genome sequencing (WGS) is a promising modality for investigation but sequence analysis methods are neither standardised, nor agreed. We sought to d...