Unraveling the Uncharacterized Domain of Carocin S2: A Ribonuclease Pectobacterium carotovorum subsp. carotovorum Bacteriocin

• Published in: Microorganisms (Basel) Vol. 10; no. 2; p. 359
• Main Authors: Chung, Ping-Chen, Lagitnay, Ruchi Briam James S., Derilo, Reymund C., Wu, Jian-Li, Chuang, Yutin, Lin, Jia-De, Chuang, Duen-Yau
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 04.02.2022; MDPI
• Subjects: active sites; antibacterial; Antibiotics; Antimicrobial agents; Bacteria; bacteriocin; Bacteriocins; Binding sites; C-Terminus; CaroS2I; CaroS2K; Circular dichroism; circular dichroism spectroscopy; Cloning; Cytotoxicity; Dichroism; DNA; Domains; E coli; Fluorescence; functional analysis; genes; Gram-negative bacteria; Immunity; immunity protein; Immunoprecipitation; Low molecular weights; Molecular weight; Mutagenesis; mutants; Pectobacterium carotovorum; Plasmids; precipitin tests; Protein expression; Protein structure; Proteins; Residues; ribonucleases; Ribonucleic acid; ribosomal RNA; RNA; rRNA; Secondary structure; Spectroscopy; Tryptophan; United States > US; CaroS2K; immunity protein; killer protein; functional analysis; antibacterial; Pectobacterium carotovorum subsp. carotovorum; bacteriocin; CaroS2I

Carocin S2 is a bacteriocin with a low molecular weight generated by Pectobacterium carotovorum subsp. carotovorum 3F3 strain. The caroS2K gene, which is found in the genomic DNA alongside the caroS2I gene, which codes for an immunity protein, encodes this bacteriocin. We explored the residues respo...