Long-Read Sequencing and Hybrid Assembly for Genomic Analysis of Clinical Brucella melitensis Isolates

• Published in: Microorganisms (Basel) Vol. 10; no. 3; p. 619
• Main Authors: Craddock, Hillary A., Motro, Yair, Zilberman, Bar, Khalfin, Boris, Bardenstein, Svetlana, Moran-Gilad, Jacob
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.03.2022; MDPI
• Subjects: Assemblies; Assembly; Brucella melitensis; Brucellosis; Case studies; clinical genomics; computer simulation; Epidemics; Epidemiology; etiological agents; Etiology; Gene sequencing; Genes; Genomes; Genomic analysis; Genomics; Israel; Laboratories; nanopores; Outer membrane proteins; Phylogeny; Virulence; Virulence factors; whole-genome sequencing; United Kingdom > UK; United States > US; Israel; clinical genomics; whole-genome sequencing; brucellosis

Brucella melitensis is a key etiological agent of brucellosis and has been increasingly subject to characterization using sequencing methodologies. This study aimed to investigate and compare short-read, long-read, and hybrid assemblies of B. melitensis. Eighteen B. melitensis isolates from Southern...