In situ structure of actin remodeling during glucose-stimulated insulin secretion using cryo-electron tomography

• Published in: Nature communications Vol. 15; no. 1; p. 1311
• Main Authors: Li, Weimin, Li, Angdi, Yu, Bing, Zhang, Xiaoxiao, Liu, Xiaoyan, White, Kate L., Stevens, Raymond C., Baumeister, Wolfgang, Sali, Andrej, Jasnin, Marion, Sun, Liping
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.02.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 13/109; 14/1; 14/28; 14/35; 14/63; 631/535/1258/1260; 631/80/128/1276; 631/80/313/2376; Actin; Actin Cytoskeleton - metabolism; Actins - metabolism; Beta cells; Depolymerization; Electron Microscope Tomography; Filaments; Glucose; Glucose - metabolism; Granular materials; Humanities and Social Sciences; Insulin; Insulin - metabolism; Insulin Secretion; Insulin-Secreting Cells - metabolism; Membranes; Microtubules; multidisciplinary; Pancreas; Science; Science (multidisciplinary); Secretion; Secretory vesicles

Actin mediates insulin secretion in pancreatic β-cells through remodeling. Hampered by limited resolution, previous studies have offered an ambiguous depiction as depolymerization and repolymerization. We report the in situ structure of actin remodeling in INS-1E β-cells during glucose-stimulated in...