Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli

• Published in: NPJ systems biology and applications Vol. 10; no. 1; pp. 109 - 14
• Main Authors: Khanijou, Jasmeet Kaur, Hee, Yan Ting, Scipion, Clement P. M., Chen, Xixian, Selvarajoo, Kumar
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/553/2695; 631/553/318; Alcohol dehydrogenase; Aldehyde dehydrogenase; Aldehyde Dehydrogenase - genetics; Aldehyde Dehydrogenase - metabolism; Bioinformatics; Biology; Biomedical and Life Sciences; Computational Biology/Bioinformatics; Computer Appl. in Life Sciences; Computer Simulation; Dehydrogenases; E coli; Enzymes; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Hexokinase; L-Lactate dehydrogenase; Life Sciences; Limonene; Limonene - metabolism; Metabolic Engineering - methods; Metabolic networks; Metabolic Networks and Pathways - genetics; Metabolomics; Metabolomics - methods; Mevalonate pathway; Mevalonic Acid - metabolism; Models, Biological; Systems Biology; Systems Biology - methods; Terpenes - metabolism
• ISSN: 2056-7189; 2056-7189
• DOI: 10.1038/s41540-024-00440-7

Engineered microorganisms have emerged as viable alternatives for limonene production. However, issues such as low enzyme abundance or activities, and regulatory feedback/forward inhibition may reduce yields. To understand the underlying metabolism, we adopted a systems biology approach for an engin...