Microsatellite Analysis of Expressed Sequence Tags and Development of EST-SSR Markers for Melampsora spp

• Published in: Plant Diseases and Pests(植物病虫害研究：英文版) Vol. 4; no. 6; pp. 21 - 26
• Main Authors: Zhibing, Wan, Min, Liu, Wanxu, Yang, Xinye, Zhang, Tongming, Yin
• Format: Journal Article
• Language: English
• Published: Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.12.2013
• Subjects: EST序列; SSR位点; SSR引物; 三核苷酸重复; 固态继电器; 微卫星分析; 表达序列标签; 重复序列

In this study,64 498 ESTs of Melampsoraspp.was assembled into 1 998 contigs,and 604SSR loci were detected on these contigs,with 736.6 bp containing one SSR on average.Among these SSRs,trinucleotide repeats were the most abundant repeats（44.70%）.As for the composition of microsatellites, AC and AT re...