Ion currents through Kir potassium channels are gated by anionic lipids

• Published in: Nature communications Vol. 13; no. 1; p. 490
• Main Authors: Jin, Ruitao, He, Sitong, Black, Katrina A., Clarke, Oliver B., Wu, Di, Bolla, Jani R., Johnson, Paul, Periasamy, Agalya, Wardak, Ahmad, Czabotar, Peter, Colman, Peter M., Robinson, Carol V., Laver, Derek, Smith, Brian J., Gulbis, Jacqueline M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.01.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 101/58; 631/45/269/1151; 631/535/1266; 631/535/1267; 82/83; 9/97; Anions - chemistry; Anions - metabolism; Binding Sites; Channels; Conduction; Crystallography, X-Ray; Grooves; Humanities and Social Sciences; Humans; Ion Channel Gating; Ion currents; Ion Transport; Lipids; Liposomes - chemistry; Liposomes - metabolism; Membrane Lipids - chemistry; Membrane Lipids - metabolism; Molecular Dynamics Simulation; multidisciplinary; Mutation; Penetration; Phosphatidylcholines - chemistry; Phosphatidylcholines - metabolism; Phosphatidylserines - chemistry; Phosphatidylserines - metabolism; Phospholipids; Plugs; Potassium; Potassium - metabolism; Potassium channels (inwardly-rectifying); Potassium Channels, Inwardly Rectifying - chemistry; Potassium Channels, Inwardly Rectifying - genetics; Potassium Channels, Inwardly Rectifying - metabolism; Regulatory sequences; Science; Science (multidisciplinary); Walls
• DOI: 10.1038/s41467-022-28148-4
• ISSN: 2041-1723

Ion currents through potassium channels are gated. Constriction of the ion conduction pathway at the inner helix bundle, the textbook gate of Kir potassium channels, has been shown to be an ineffective permeation control, creating a rift in our understanding of how these channels are gated. Here we present evidence that anionic lipids act as interactive response elements sufficient to gate potassium conduction. We demonstrate the limiting barrier to K + permeation lies within the ion conduction pathway and show that this gate is operated by the fatty acyl tails of lipids that infiltrate the conduction pathway via fenestrations in the walls of the pore. Acyl tails occupying a surface groove extending from the cytosolic interface to the conduction pathway provide a potential means of relaying cellular signals, mediated by anionic lipid head groups bound at the canonical lipid binding site, to the internal gate. The Kir potassium channels are known to operate and gate without a major conformational change. Here, the authors identify the permeation gate of Kir channels as a steric plug within the conduction pathway, describing how tightly associated anionic lipids pushing into fenestrations in the pore walls engage with the plug to operate the gate.