Engineering circular RNA for enhanced protein production

• Published in: Nature biotechnology Vol. 41; no. 2; pp. 262 - 272
• Main Authors: Chen, Robert, Wang, Sean K., Belk, Julia A., Amaya, Laura, Li, Zhijian, Cardenas, Angel, Abe, Brian T., Chen, Chun-Kan, Wender, Paul A., Chang, Howard Y.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.02.2023; Nature Publishing Group
• Subjects: 3' Untranslated regions; 631/1647/2300; 631/208/1792; 631/61/201; 631/61/51/391; Agriculture; Aptamers; Bioinformatics; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Circular RNA; Cloning; Design optimization; Gene expression; In vivo methods and tests; Life Sciences; mRNA; Protein folding; Proteins; Ribonucleic acid; RNA; RNA - genetics; RNA - metabolism; RNA Splicing; RNA, Circular - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Severe acute respiratory syndrome coronavirus 2; Splicing; Topology; Transgenes; Translation; Translation initiation

Circular RNAs (circRNAs) are stable and prevalent RNAs in eukaryotic cells that arise from back-splicing. Synthetic circRNAs and some endogenous circRNAs can encode proteins, raising the promise of circRNA as a platform for gene expression. In this study, we developed a systematic approach for rapid...