Engineering a riboswitch-based genetic platform for the self-directed evolution of acid-tolerant phenotypes

• Published in: Nature communications Vol. 8; no. 1; pp. 411 - 12
• Main Authors: Pham, Hoang Long, Wong, Adison, Chua, Niying, Teo, Wei Suong, Yew, Wen Shan, Chang, Matthew Wook
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 04.09.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 631/553/552; 631/61; Acids - pharmacology; Biological evolution; Directed evolution; Directed Molecular Evolution; Engineering; Escherichia coli - genetics; Gene expression; Genetic Engineering - methods; Genotype; Humanities and Social Sciences; Hydrogen ions; Hydrogen-Ion Concentration; Metabolism; multidisciplinary; Mutation - genetics; Organic acids; pH effects; Phenotype; Riboswitch - genetics; Riboswitches; Science; Science (multidisciplinary)

Environmental pH is a fundamental signal continuously directing the metabolism and behavior of living cells. Programming the precise cellular response toward environmental pH is, therefore, crucial for engineering cells for increasingly sophisticated functions. Herein, we engineer a set of riboswitc...