Immunohistochemical localization of PDE10A in the rat brain

• Published in: Brain research Vol. 985; no. 2; pp. 113 - 126
• Main Authors: Seeger, Thomas F, Bartlett, Brenda, Coskran, Timothy M, Culp, Jeffrey S, James, Larry C, Krull, David L, Lanfear, Jerry, Ryan, Anne M, Schmidt, Christopher J, Strick, Christine A, Varghese, Alison H, Williams, Robert D, Wylie, Patricia G, Menniti, Frank S
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 26.09.2003; Amsterdam Elsevier; New York, NY
• Subjects: Anatomy; Animals; Antibodies, Monoclonal - metabolism; Autoradiography; Biological and medical sciences; Blotting, Western; Brain - cytology; Brain - metabolism; Brain Chemistry; Cell Line; Central nervous system; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; DNA, Complementary - chemistry; DNA, Complementary - genetics; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Immunohistochemistry - methods; In Situ Hybridization; Insecta; Localization; Male; Medium spiny neuron; Mice; Mice, Inbred BALB C; Neurons - cytology; Neurons - metabolism; Oligodeoxyribonucleotides, Antisense; PDE10A; Peptide Fragments - genetics; Peptide Fragments - immunology; Peptide Fragments - metabolism; Phosphodiesterase; Phosphoric Diester Hydrolases - genetics; Phosphoric Diester Hydrolases - immunology; Phosphoric Diester Hydrolases - metabolism; Phosphorus Isotopes - pharmacokinetics; Precipitin Tests; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - metabolism; Striatum; Transfection; Vertebrates: nervous system and sense organs; Medium spiny neuron; Immunohistochemistry; Striatum; PDE10A; Localization; In situ hybridization; Neurotransmitters, modulators, transporters, and receptors; Phosphodiesterase; and receptors Second messengers and phosphorylation PDE10A; Phosphodiesterase; Striatum; Medium spiny neuron; Localization; Immunohistochemistry; In situ hybridization; Rat; transporters; Enzyme; Rodentia; Central nervous system; Vertebrata; Neurotransmitters; Mammalia; modulators; Brain (vertebrata)

PDE10A is a newly identified cAMP/cGMP phosphodiesterase for which mRNA is highly expressed in the mammalian striatum. In the present study, PDE10A protein and mRNA expression throughout the rat brain were determined, using a monoclonal antibody (24F3.F11) for Western blot and immunohistochemical an...