Functional and structural characterization of a two-MAb cocktail for delayed treatment of enterovirus D68 infections

• Published in: Nature communications Vol. 12; no. 1; pp. 2904 - 16
• Main Authors: Zhang, Chao, Xu, Cong, Dai, Wenlong, Wang, Yifan, Liu, Zhi, Zhang, Xueyang, Wang, Xuesong, Wang, Haikun, Gong, Sitang, Cong, Yao, Huang, Zhong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.05.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/1; 147/28; 631/154; 631/326/596; 631/535; 692/699/255/2514; Acids; Animals; Antibodies; Antibodies, Monoclonal - administration & dosage; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - metabolism; Antibodies, Neutralizing - administration & dosage; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - metabolism; Antigens; Binding; Binding sites; Canyons; Cell Line, Tumor; Chemical compounds; Cryoelectron Microscopy; Enterovirus D, Human - drug effects; Enterovirus D, Human - immunology; Enterovirus D, Human - physiology; Enterovirus Infections - drug therapy; Enterovirus Infections - immunology; Enterovirus Infections - virology; Enteroviruses; Epidemics; Female; Humanities and Social Sciences; Humans; Infections; Laboratories; Mice; Mice, Inbred BALB C; Monoclonal antibodies; multidisciplinary; Myelitis; Neutralization; Neutralizing; Pharmacology; Protein Binding - drug effects; Receptors; Receptors, Cell Surface - immunology; Receptors, Cell Surface - metabolism; Respiratory diseases; Science; Science (multidisciplinary); Signs and symptoms; Steric hindrance; Structural analysis; Structure-function relationships; Time-to-Treatment; Treatment Outcome; Uncoating; Virion - drug effects; Virion - immunology; Virion - metabolism; Virion - ultrastructure; Virions; Virus Uncoating - drug effects; Virus-like particles

Enterovirus D68 (EV-D68) is an emerging pathogen associated with respiratory diseases and/or acute flaccid myelitis. Here, two MAbs, 2H12 and 8F12, raised against EV-D68 virus-like particle (VLP), show distinct preference in binding VLP and virion and in neutralizing different EV-D68 strains. A comb...