Magnetic hydrogel particles improve nanopore sequencing of SARS-CoV-2 and other respiratory viruses

• Published in: Scientific reports Vol. 13; no. 1; p. 2163
• Main Authors: Andersen, P., Barksdale, S., Barclay, R.A., Smith, N., Fernandes, J., Besse, K., Goldfarb, D., Barbero, R., Dunlap, R., Jones-Roe, T., Kelly, R., Miao, S., Ruhunusiri, C., Munns, A., Mosavi, S., Sanson, L., Munns, D., Sahoo, S., Swahn, O., Hull, K., White, D., Kolb, K., Noroozi, F., Seelam, J., Patnaik, A., Lepene, B.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.02.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114; 631/1647; 631/1647/2163; 631/1647/514; 631/337; 631/61; 692/4017; 692/53; 692/700; Acids; Aquatic birds; Automation; Coronaviruses; COVID-19; Disease transmission; High-Throughput Nucleotide Sequencing - methods; Humanities and Social Sciences; Humans; Hydrogels; Influenza; Life sciences; Magnetic Phenomena; multidisciplinary; Mutation; Nanopore Sequencing - methods; Respiratory syncytial virus; SARS-CoV-2; Science; Science (multidisciplinary); Severe acute respiratory syndrome coronavirus 2; Viruses

Presented here is a magnetic hydrogel particle enabled workflow for capturing and concentrating SARS-CoV-2 from diagnostic remnant swab samples that significantly improves sequencing results using the Oxford Nanopore Technologies MinION sequencing platform. Our approach utilizes a novel affinity-bas...