Utilization of rare codon-rich markers for screening amino acid overproducers

• Published in: Nature communications Vol. 9; no. 1; pp. 3616 - 11
• Main Authors: Zheng, Bo, Ma, Xiaoyan, Wang, Ning, Ding, Tingting, Guo, Liwei, Zhang, Xiaorong, Yang, Yu, Li, Chun, Huo, Yi-Xin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.09.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 49; 49/47; 631/1647/2163; 631/326/2522; 631/326/41/2537; 631/61/318; 82/80; Amino acid starvation; Amino acids; Amino Acids - biosynthesis; Amino Acids - genetics; Antibiotic resistance; Antibiotics; Arginine; Codon; Codons; Corynebacterium glutamicum; Corynebacterium glutamicum - genetics; Corynebacterium glutamicum - growth & development; Corynebacterium glutamicum - metabolism; E coli; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - metabolism; Frequency dependence; Genetic Engineering - methods; Genetic Markers; Green Fluorescent Proteins - genetics; Humanities and Social Sciences; L-Serine; Leucine; Markers; Microorganisms, Genetically-Modified; multidisciplinary; Proteins; Science; Science (multidisciplinary); Screening; Starvation; Translation; Translations

The translation of rare codons relies on their corresponding rare tRNAs, which could not be fully charged under amino acid starvation. Theoretically, disrupted or retarded translation caused by the lack of charged rare tRNAs can be partially restored by feeding or intracellular synthesis of the corr...