Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates

• Published in: Nature communications Vol. 14; no. 1; p. 455
• Main Authors: Park, Daehun, Wu, Yumei, Wang, Xinbo, Gowrishankar, Swetha, Baublis, Aaron, De Camilli, Pietro
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.01.2023; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 14/19; 14/28; 14/35; 631/378/340; 631/378/87; 82/58; 82/80; 82/83; Autophagy; Clustering; Condensates; Ectopic expression; Endocytosis; Fibroblasts; Humanities and Social Sciences; Membrane proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; Membranes; multidisciplinary; Nerve endings; Proteins; Science; Science (multidisciplinary); Synapses; Synapses - metabolism; Synapsin; Synapsins - genetics; Synapsins - metabolism; Synaptic vesicles; Synaptic Vesicles - metabolism; Synaptophysin; Synaptophysin - metabolism; Vesicles

Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. Here we show that unlike synaptophysin, other major integral SV membrane proteins fail to form con...