Ultrasensitive multiplexed immunoassay of autophagic biomarkers based on Au/rGO and Au nanocages amplifying electrochemcial signal

• Published in: Scientific reports Vol. 7; no. 1; pp. 2442 - 10
• Main Authors: Wang, Guannan, Li, Yankun, Liu, Jinlei, Yuan, Yajing, Shen, Zhaoliang, Mei, Xifan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.05.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 639/301/357/918/1052; 639/925/929; Antibodies; Autophagy; Beclin-1 - analysis; Binding sites; Biomarkers; Biomarkers - analysis; Biosensing Techniques - methods; Electric Conductivity; Electrochemical Techniques - methods; Enzyme-linked immunosorbent assay; Gold - chemistry; Humanities and Social Sciences; Humans; Immunoassay - methods; Lysates; Metal Nanoparticles - chemistry; Microtubule-Associated Proteins - analysis; multidisciplinary; Nanocomposites - chemistry; Oxides - chemistry; Phagocytosis; Reproducibility of Results; Science; Science (multidisciplinary); Synesthesia

A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding sites for...