Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles

• Published in: Nature communications Vol. 9; no. 1; pp. 3290 - 6
• Main Authors: Chen, Chaohao, Wang, Fan, Wen, Shihui, Su, Qian Peter, Wu, Mike C. L., Liu, Yongtao, Wang, Baoming, Li, Du, Shan, Xuchen, Kianinia, Mehran, Aharonovich, Igor, Toth, Milos, Jackson, Shaun P., Xi, Peng, Jin, Dayong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.08.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 14/69; 140/125; 639/301/357/354; 639/624/1107/328/2057; 639/624/1107/328/2238; Emissions; Excitation; Femtosecond pulsed lasers; Fluorescence; Fluorescence microscopy; Humanities and Social Sciences; I.R. radiation; Image resolution; Laser beams; Lasers; Liver; multidisciplinary; Nanoparticles; Near infrared radiation; Penetration depth; Phototoxicity; Saturation; Science; Science (multidisciplinary); Stimulated emission; Synchronism; Synchronization; Upconversion

Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spati...