Identifying transposable element expression dynamics and heterogeneity during development at the single-cell level with a processing pipeline scTE

• Published in: Nature communications Vol. 12; no. 1; pp. 1456 - 14
• Main Authors: He, Jiangping, Babarinde, Isaac A., Sun, Li, Xu, Shuyang, Chen, Ruhai, Shi, Junjie, Wei, Yuanjie, Li, Yuhao, Ma, Gang, Zhuang, Qiang, Hutchins, Andrew P., Chen, Jiekai
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.03.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/100; 38/91; 631/136/142; 631/1647/48; 631/61/514/1949; Animals; Cell fate; Chromatin; DNA Transposable Elements - genetics; Embryo cells; Embryogenesis; Embryonic growth stage; Embryonic Stem Cells; Gastrulation; Gene expression; Gene Expression Regulation, Developmental; Genetic Heterogeneity; Genomes; Heterogeneity; Humanities and Social Sciences; Humans; Mice; multidisciplinary; Organogenesis - genetics; Pipelines; Pluripotency; Science; Science (multidisciplinary); Single-Cell Analysis - methods; Somatic cells; Stem cells; Subpopulations; Transposons

Transposable elements (TEs) make up a majority of a typical eukaryote’s genome, and contribute to cell heterogeneity in unclear ways. Single-cell sequencing technologies are powerful tools to explore cells, however analysis is typically gene-centric and TE expression has not been addressed. Here, we...