Perturbed cholesterol and vesicular trafficking associated with dengue blocking in Wolbachia-infected Aedes aegypti cells

• Published in: Nature communications Vol. 8; no. 1; pp. 526 - 10
• Main Authors: Geoghegan, Vincent, Stainton, Kirsty, Rainey, Stephanie M., Ant, Thomas H., Dowle, Adam A., Larson, Tony, Hester, Svenja, Charles, Philip D., Thomas, Benjamin, Sinkins, Steven P.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.09.2017; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/41/2533; 631/326/596/1413; 631/45/287; 631/45/475; Accumulation; Aedes - metabolism; Aedes - microbiology; Aedes - virology; Aedes aegypti; Animals; Aquatic insects; Bacteria; Blocking; Cholesterol; Cholesterol - metabolism; Cyclodextrins; Cytoplasmic Vesicles - metabolism; Dengue - transmission; Dengue - virology; Dengue fever; Dengue Virus - physiology; Disease control; Endoplasmic reticulum; Esterification; Genetic disorders; Homeostasis; Humanities and Social Sciences; Humans; Infections; Inhibition; Intracellular; Lipid metabolism; Lipids; Metabolism; Midgut; Mosquitoes; multidisciplinary; Proteomics; Replication; Science; Science (multidisciplinary); Vector-borne diseases; Viral diseases; Viruses; Wolbachia; Wolbachia - physiology; β-Cyclodextrin

Wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. To better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the...