Multiplex base- and prime-editing with drive-and-process CRISPR arrays

Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multi...

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Published inNature communications Vol. 13; no. 1; pp. 2771 - 13
Main Authors Yuan, Qichen, Gao, Xue
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 19.05.2022
Nature Publishing Group
Nature Portfolio
Subjects
45/22
45/23
45/29
45/91
631/208
631/337/4041/3196
631/61
Arrays
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
Constraining
CRISPR
CRISPR-Cas Systems - genetics
DNA-directed RNA polymerase
Editing
Gene Editing
Gene expression
Gene loci
Genomics
gRNA
Humanities and Social Sciences
Humans
Medical research
multidisciplinary
Multiplexing
RNA polymerase
RNA, Guide, CRISPR-Cas Systems - genetics
RNA, Transfer - genetics
Science
Science (multidisciplinary)
Therapeutic applications
Transfer RNA
tRNA Cys
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Abstract Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex prime-editing (MPE) in human cells. We leverage tRNA as the RNA polymerase III promoter to drive the expression of tandemly assembled tRNA-guide RNA (gRNA) arrays, of which the individual gRNAs are released by the cellular endogenous tRNA processing machinery. We engineer a 75-nt human cysteine tRNA (hCtRNA) for the DAP array, achieving up to 31-loci MBE and up to 3-loci MPE. By applying MBE or MPE elements for deliveries via adeno-associated virus (AAV) and lentivirus, we demonstrate simultaneous editing of multiple disease-relevant genomic loci. Our work streamlines the expression and processing of gRNAs on a single array and establishes efficient MBE and MPE strategies for biomedical research and therapeutic applications. Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here the authors describe drive-and-process CRISPR array architectures for multiplex base-editing and multiplex prime-editing in human cells.
AbstractList Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex prime-editing (MPE) in human cells. We leverage tRNA as the RNA polymerase III promoter to drive the expression of tandemly assembled tRNA-guide RNA (gRNA) arrays, of which the individual gRNAs are released by the cellular endogenous tRNA processing machinery. We engineer a 75-nt human cysteine tRNA (hCtRNA) for the DAP array, achieving up to 31-loci MBE and up to 3-loci MPE. By applying MBE or MPE elements for deliveries via adeno-associated virus (AAV) and lentivirus, we demonstrate simultaneous editing of multiple disease-relevant genomic loci. Our work streamlines the expression and processing of gRNAs on a single array and establishes efficient MBE and MPE strategies for biomedical research and therapeutic applications.
Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex prime-editing (MPE) in human cells. We leverage tRNA as the RNA polymerase III promoter to drive the expression of tandemly assembled tRNA-guide RNA (gRNA) arrays, of which the individual gRNAs are released by the cellular endogenous tRNA processing machinery. We engineer a 75-nt human cysteine tRNA (hCtRNA) for the DAP array, achieving up to 31-loci MBE and up to 3-loci MPE. By applying MBE or MPE elements for deliveries via adeno-associated virus (AAV) and lentivirus, we demonstrate simultaneous editing of multiple disease-relevant genomic loci. Our work streamlines the expression and processing of gRNAs on a single array and establishes efficient MBE and MPE strategies for biomedical research and therapeutic applications.Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex prime-editing (MPE) in human cells. We leverage tRNA as the RNA polymerase III promoter to drive the expression of tandemly assembled tRNA-guide RNA (gRNA) arrays, of which the individual gRNAs are released by the cellular endogenous tRNA processing machinery. We engineer a 75-nt human cysteine tRNA (hCtRNA) for the DAP array, achieving up to 31-loci MBE and up to 3-loci MPE. By applying MBE or MPE elements for deliveries via adeno-associated virus (AAV) and lentivirus, we demonstrate simultaneous editing of multiple disease-relevant genomic loci. Our work streamlines the expression and processing of gRNAs on a single array and establishes efficient MBE and MPE strategies for biomedical research and therapeutic applications.
Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex prime-editing (MPE) in human cells. We leverage tRNA as the RNA polymerase III promoter to drive the expression of tandemly assembled tRNA-guide RNA (gRNA) arrays, of which the individual gRNAs are released by the cellular endogenous tRNA processing machinery. We engineer a 75-nt human cysteine tRNA (hCtRNA) for the DAP array, achieving up to 31-loci MBE and up to 3-loci MPE. By applying MBE or MPE elements for deliveries via adeno-associated virus (AAV) and lentivirus, we demonstrate simultaneous editing of multiple disease-relevant genomic loci. Our work streamlines the expression and processing of gRNAs on a single array and establishes efficient MBE and MPE strategies for biomedical research and therapeutic applications.Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here the authors describe drive-and-process CRISPR array architectures for multiplex base-editing and multiplex prime-editing in human cells.
Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here the authors describe drive-and-process CRISPR array architectures for multiplex base-editing and multiplex prime-editing in human cells.
Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here, we describe drive-and-process (DAP) CRISPR array architectures for multiplex base-editing (MBE) and multiplex prime-editing (MPE) in human cells. We leverage tRNA as the RNA polymerase III promoter to drive the expression of tandemly assembled tRNA-guide RNA (gRNA) arrays, of which the individual gRNAs are released by the cellular endogenous tRNA processing machinery. We engineer a 75-nt human cysteine tRNA (hCtRNA) for the DAP array, achieving up to 31-loci MBE and up to 3-loci MPE. By applying MBE or MPE elements for deliveries via adeno-associated virus (AAV) and lentivirus, we demonstrate simultaneous editing of multiple disease-relevant genomic loci. Our work streamlines the expression and processing of gRNAs on a single array and establishes efficient MBE and MPE strategies for biomedical research and therapeutic applications. Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex genomics and polygenic diseases. Here the authors describe drive-and-process CRISPR array architectures for multiplex base-editing and multiplex prime-editing in human cells.
ArticleNumber 2771
Author Gao, Xue
Yuan, Qichen
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Cites_doi 10.1016/j.molcel.2014.04.022
10.1038/s41586-019-1161-z
10.1074/jbc.M112.372680
10.1038/s41556-022-00870-7
10.1038/s41587-020-0561-9
10.1038/nbt.3737
10.1038/nmeth.4483
10.1038/s41587-020-0414-6
10.1038/s41586-020-03086-7
10.1016/0092-8674(81)90348-2
10.1038/s41591-020-0790-y
10.1038/nbt.3117
10.1126/science.aas9129
10.1038/s41587-020-0609-x
10.1073/pnas.1420294112
10.1038/nature24644
10.1038/nchembio.2410
10.1038/s41587-020-0453-z
10.1038/s41592-019-0508-6
10.1126/science.aba8853
10.1093/nar/gkv1309
10.1038/s41587-020-0600-6
10.1126/science.aaz6063
10.1371/journal.pone.0198714
10.1177/1087057103256465
10.1038/s41551-019-0501-5
10.1038/s41467-020-15053-x
10.1126/science.abg6155
10.1038/s41596-020-00450-9
10.1186/1743-422X-10-74
10.1038/nmeth.3047
10.1089/crispr.2018.0014
10.1016/j.bbrc.2016.11.129
10.1038/s41587-021-00938-z
10.1038/s41467-021-21337-7
10.1038/s41587-018-0011-0
10.1038/s41586-019-1711-4
10.1038/s41587-019-0032-3
10.1038/nbt.4172
10.1038/s41467-020-16173-0
10.1038/s41556-021-00836-1
10.1038/s41587-020-0491-6
10.1038/nbt.4102
10.1016/j.cell.2016.04.003
10.1093/nar/gkw413
10.1038/s41586-022-04395-9
10.1038/s41467-019-13007-6
10.1016/j.ymthe.2021.09.002
10.1038/nplants.2017.18
10.1016/j.cell.2021.12.021
10.1038/nbt.2647
10.1038/nature17945
10.1038/s41586-019-1314-0
10.1021/acschembio.7b00842
10.1038/nature17946
10.1038/s41591-019-0401-y
10.1126/sciadv.abg4910
10.1038/s41587-020-0412-8
10.1126/science.1231143
10.1038/s41598-018-31476-5
10.1261/rna.051631.115
10.1038/nprot.2017.143
10.1126/sciadv.aau0766
10.1038/s41467-022-30514-1
10.1038/s41586-022-04494-7
10.1126/science.abj4008
10.1101/2021.11.01.466786
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PublicationDecade 2020
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
PublicationTitle Nature communications
PublicationTitleAbbrev Nat Commun
PublicationTitleAlternate Nat Commun
PublicationYear 2022
Publisher Nature Publishing Group UK
Nature Publishing Group
Nature Portfolio
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Publishing Group
– name: Nature Portfolio
References Walton, Christie, Whittaker, Kleinstiver (CR26) 2020; 368
Zhong, Wang, Li, Tran, Farzan (CR17) 2017; 13
Wu (CR45) 2019; 25
Nissim (CR55) 2014; 54
Tak (CR18) 2017; 14
Chan, Lowe (CR33) 2016; 44
Miller (CR25) 2020; 38
Hsu (CR67) 2021; 12
Anzalone, Koblan, Liu (CR6) 2020; 38
Anzalone (CR3) 2019; 576
Zeng (CR46) 2020; 26
Grünewald (CR36) 2019; 569
Campa, Weisbach, Santinha, Incarnato, Platt (CR19) 2019; 16
Koblan (CR35) 2018; 36
Koblan (CR4) 2021; 39
Lowe, Chan (CR32) 2016; 44
Tsai (CR44) 2015; 33
Kleinstiver (CR29) 2019; 37
Thompson (CR56) 2018; 13
Lundholt, Scudder, Pagliaro (CR66) 2003; 8
Segel (CR63) 2021; 373
Gaudelli (CR13) 2020; 38
Hofstetter, Kressmann, Birnstiel (CR37) 1981; 24
Kluesner (CR40) 2018; 1
Tang (CR27) 2017; 3
Doman, Raguram, Newby, Liu (CR42) 2020; 38
Sanjana, Shalem, Zhang (CR51) 2014; 11
McCarty, Graham, Studená, Ledesma-Amaro (CR11) 2020; 11
Fonfara, Richter, Bratovič, Le Rhun, Charpentier (CR15) 2016; 532
Zhou (CR38) 2019; 571
Dong, Xie, Chen, Yang, Mao (CR53) 2017; 482
Chemello (CR9) 2021; 7
Li (CR28) 2018; 36
Bak, Dever, Porteus (CR49) 2018; 13
Guo (CR21) 2022; 24
Jin (CR60) 2020; 370
Zetsche (CR16) 2017; 35
Clement (CR41) 2019; 37
Huang, Newby, Liu (CR5) 2021; 16
CR58
CR57
Xie, Minkenberg, Yang (CR31) 2015; 112
DeWeirdt (CR20) 2021; 39
Richter (CR30) 2020; 38
Hsu (CR52) 2013; 31
Yamano (CR22) 2016; 165
Levy (CR48) 2020; 4
Banskota (CR64) 2022; 185
Webber (CR12) 2019; 10
Gaudelli (CR2) 2017; 551
Ellis (CR50) 2013; 10
Kurata (CR54) 2018; 13
Mefferd, Kornepati, Bogerd, Kennedy, Cullen (CR39) 2015; 21
Cong (CR10) 2013; 339
Wang (CR59) 2022; 24
CR24
Carvajal-Vallejos, Pallissé, Mootz, Schmidt (CR47) 2012; 287
CR68
Newby, Liu (CR7) 2021; 29
Koblan (CR8) 2021; 589
Komor, Kim, Packer, Zuris, Liu (CR1) 2016; 533
CR62
CR61
Nishimasu (CR23) 2018; 361
Shiraki, Kawakami (CR34) 2018; 8
Li (CR65) 2022; 602
Kurt (CR43) 2021; 39
Zhang (CR14) 2020; 11
BL Ellis (30514_CR50) 2013; 10
T Yamano (30514_CR22) 2016; 165
PP Chan (30514_CR33) 2016; 44
RO Bak (30514_CR49) 2018; 13
C Zhou (30514_CR38) 2019; 571
J Grünewald (30514_CR36) 2019; 569
S Banskota (30514_CR64) 2022; 185
P Carvajal-Vallejos (30514_CR47) 2012; 287
M Kurata (30514_CR54) 2018; 13
JL Doman (30514_CR42) 2020; 38
SQ Tsai (30514_CR44) 2015; 33
H Nishimasu (30514_CR23) 2018; 361
I Fonfara (30514_CR15) 2016; 532
YE Tak (30514_CR18) 2017; 14
CC Campa (30514_CR19) 2019; 16
RT Walton (30514_CR26) 2020; 368
Y Wu (30514_CR45) 2019; 25
30514_CR24
X Tang (30514_CR27) 2017; 3
30514_CR68
LW Koblan (30514_CR35) 2018; 36
H Hofstetter (30514_CR37) 1981; 24
W Zhang (30514_CR14) 2020; 11
DB Thompson (30514_CR56) 2018; 13
T Shiraki (30514_CR34) 2018; 8
PD Hsu (30514_CR52) 2013; 31
IC Kurt (30514_CR43) 2021; 39
TP Huang (30514_CR5) 2021; 16
S Li (30514_CR65) 2022; 602
30514_CR61
30514_CR62
B Zetsche (30514_CR16) 2017; 35
SM Miller (30514_CR25) 2020; 38
LY Guo (30514_CR21) 2022; 24
AC Komor (30514_CR1) 2016; 533
AV Anzalone (30514_CR6) 2020; 38
BR Webber (30514_CR12) 2019; 10
J Zeng (30514_CR46) 2020; 26
GA Newby (30514_CR7) 2021; 29
30514_CR57
30514_CR58
X Li (30514_CR28) 2018; 36
BP Kleinstiver (30514_CR29) 2019; 37
M Segel (30514_CR63) 2021; 373
F Chemello (30514_CR9) 2021; 7
L Nissim (30514_CR55) 2014; 54
LW Koblan (30514_CR8) 2021; 589
NM Gaudelli (30514_CR2) 2017; 551
JM Levy (30514_CR48) 2020; 4
LW Koblan (30514_CR4) 2021; 39
AL Mefferd (30514_CR39) 2015; 21
K Clement (30514_CR41) 2019; 37
MF Richter (30514_CR30) 2020; 38
AV Anzalone (30514_CR3) 2019; 576
PC DeWeirdt (30514_CR20) 2021; 39
NS McCarty (30514_CR11) 2020; 11
BK Lundholt (30514_CR66) 2003; 8
NM Gaudelli (30514_CR13) 2020; 38
JY Hsu (30514_CR67) 2021; 12
F Dong (30514_CR53) 2017; 482
G Zhong (30514_CR17) 2017; 13
NE Sanjana (30514_CR51) 2014; 11
TM Lowe (30514_CR32) 2016; 44
C Wang (30514_CR59) 2022; 24
MG Kluesner (30514_CR40) 2018; 1
L Cong (30514_CR10) 2013; 339
K Xie (30514_CR31) 2015; 112
X Jin (30514_CR60) 2020; 370
References_xml – volume: 54
  start-page: 698
  year: 2014
  end-page: 710
  ident: CR55
  article-title: Multiplexed and Programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2014.04.022
– volume: 569
  start-page: 433
  year: 2019
  end-page: 437
  ident: CR36
  article-title: Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors
  publication-title: Nature
  doi: 10.1038/s41586-019-1161-z
– volume: 287
  start-page: 28686
  year: 2012
  end-page: 28696
  ident: CR47
  article-title: Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M112.372680
– volume: 24
  start-page: 590
  year: 2022
  end-page: 600
  ident: CR21
  article-title: Multiplexed genome regulation in vivo with hyper-efficient Cas12a
  publication-title: Nat. Cell Biol.
  doi: 10.1038/s41556-022-00870-7
– ident: CR68
– volume: 38
  start-page: 824
  year: 2020
  end-page: 844
  ident: CR6
  article-title: Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0561-9
– volume: 35
  start-page: 31
  year: 2017
  end-page: 34
  ident: CR16
  article-title: Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.3737
– volume: 14
  start-page: 1163
  year: 2017
  end-page: 1166
  ident: CR18
  article-title: Inducible and multiplex gene regulation using CRISPR–Cpf1-based transcription factors
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.4483
– volume: 38
  start-page: 620
  year: 2020
  end-page: 628
  ident: CR42
  article-title: Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0414-6
– volume: 589
  start-page: 608
  year: 2021
  end-page: 614
  ident: CR8
  article-title: In vivo base editing rescues Hutchinson–Gilford progeria syndrome in mice
  publication-title: Nature
  doi: 10.1038/s41586-020-03086-7
– volume: 24
  start-page: 573
  year: 1981
  end-page: 585
  ident: CR37
  article-title: A split promoter for a eucaryotic tRNA gene
  publication-title: Cell
  doi: 10.1016/0092-8674(81)90348-2
– volume: 26
  start-page: 535
  year: 2020
  end-page: 541
  ident: CR46
  article-title: Therapeutic base editing of human hematopoietic stem cells
  publication-title: Nat. Med.
  doi: 10.1038/s41591-020-0790-y
– ident: CR61
– volume: 33
  start-page: 187
  year: 2015
  end-page: 197
  ident: CR44
  article-title: GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.3117
– ident: CR58
– volume: 361
  start-page: 1259
  year: 2018
  ident: CR23
  article-title: Engineered CRISPR-Cas9 nuclease with expanded targeting space
  publication-title: Science
  doi: 10.1126/science.aas9129
– volume: 39
  start-page: 41
  year: 2021
  end-page: 46
  ident: CR43
  article-title: CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0609-x
– volume: 112
  start-page: 3570
  year: 2015
  end-page: 3575
  ident: CR31
  article-title: Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
  publication-title: Proc. Natl Acad. Sci.
  doi: 10.1073/pnas.1420294112
– volume: 551
  start-page: 464
  year: 2017
  end-page: 471
  ident: CR2
  article-title: Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
  publication-title: Nature
  doi: 10.1038/nature24644
– volume: 13
  start-page: 839
  year: 2017
  end-page: 841
  ident: CR17
  article-title: Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells
  publication-title: Nat. Chem. Biol.
  doi: 10.1038/nchembio.2410
– volume: 38
  start-page: 883
  year: 2020
  end-page: 891
  ident: CR30
  article-title: Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0453-z
– volume: 16
  start-page: 887
  year: 2019
  end-page: 893
  ident: CR19
  article-title: Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0508-6
– volume: 368
  start-page: 290
  year: 2020
  end-page: 296
  ident: CR26
  article-title: Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
  publication-title: Science
  doi: 10.1126/science.aba8853
– volume: 44
  start-page: D184
  year: 2016
  end-page: D189
  ident: CR33
  article-title: GtRNAdb 2.0: an expanded database of transfer RNA genes identified in complete and draft genomes
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkv1309
– volume: 39
  start-page: 94
  year: 2021
  end-page: 104
  ident: CR20
  article-title: Optimization of AsCas12a for combinatorial genetic screens in human cells
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0600-6
– volume: 370
  start-page: eaaz6063
  year: 2020
  ident: CR60
  article-title: In vivo Perturb-Seq reveals neuronal and glial abnormalities associated with autism risk genes
  publication-title: Science
  doi: 10.1126/science.aaz6063
– volume: 13
  start-page: e0198714
  year: 2018
  ident: CR54
  article-title: Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays
  publication-title: PLOS ONE
  doi: 10.1371/journal.pone.0198714
– volume: 8
  start-page: 566
  year: 2003
  end-page: 570
  ident: CR66
  article-title: A simple technique for reducing-edge effect in cell-based assays
  publication-title: J. Biomol. Screen.
  doi: 10.1177/1087057103256465
– volume: 4
  start-page: 97
  year: 2020
  end-page: 110
  ident: CR48
  article-title: Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated viruses
  publication-title: Nat. Biomed. Eng.
  doi: 10.1038/s41551-019-0501-5
– volume: 11
  year: 2020
  ident: CR11
  article-title: Multiplexed CRISPR technologies for gene editing and transcriptional regulation
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-020-15053-x
– ident: CR57
– volume: 373
  start-page: 882
  year: 2021
  end-page: 889
  ident: CR63
  article-title: Mammalian retrovirus-like protein PEG10 packages its own mRNA and can be pseudotyped for mRNA delivery
  publication-title: Science
  doi: 10.1126/science.abg6155
– volume: 16
  start-page: 1089
  year: 2021
  end-page: 1128
  ident: CR5
  article-title: Precision genome editing using cytosine and adenine base editors in mammalian cells
  publication-title: Nat. Protoc.
  doi: 10.1038/s41596-020-00450-9
– volume: 10
  year: 2013
  ident: CR50
  article-title: A survey of ex vivo/in vitro transduction efficiency of mammalian primary cells and cell lines with Nine natural adeno-associated virus (AAV1-9) and one engineered adeno-associated virus serotype
  publication-title: Virol. J.
  doi: 10.1186/1743-422X-10-74
– volume: 11
  start-page: 783
  year: 2014
  end-page: 784
  ident: CR51
  article-title: Improved vectors and genome-wide libraries for CRISPR screening
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3047
– volume: 1
  start-page: 239
  year: 2018
  end-page: 250
  ident: CR40
  article-title: EditR: A method to quantify base editing from Sanger sequencing
  publication-title: CRISPR J.
  doi: 10.1089/crispr.2018.0014
– volume: 482
  start-page: 889
  year: 2017
  end-page: 895
  ident: CR53
  article-title: Polycistronic tRNA and CRISPR guide-RNA enables highly efficient multiplexed genome engineering in human cells
  publication-title: Biochem. Biophys. Res. Commun.
  doi: 10.1016/j.bbrc.2016.11.129
– volume: 39
  start-page: 1414
  year: 2021
  end-page: 1425
  ident: CR4
  article-title: Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-021-00938-z
– volume: 12
  start-page: 1
  year: 2021
  end-page: 6
  ident: CR67
  article-title: PrimeDesign software for rapid and simplified design of prime editing guide RNAs
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-021-21337-7
– volume: 37
  start-page: 276
  year: 2019
  end-page: 282
  ident: CR29
  article-title: Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-018-0011-0
– volume: 576
  start-page: 149
  year: 2019
  end-page: 157
  ident: CR3
  article-title: Search-and-replace genome editing without double-strand breaks or donor DNA
  publication-title: Nature
  doi: 10.1038/s41586-019-1711-4
– volume: 37
  start-page: 224
  year: 2019
  end-page: 226
  ident: CR41
  article-title: CRISPResso2 provides accurate and rapid genome editing sequence analysis
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-019-0032-3
– volume: 36
  start-page: 843
  year: 2018
  end-page: 846
  ident: CR35
  article-title: Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.4172
– volume: 11
  year: 2020
  ident: CR14
  article-title: Multiplex precise base editing in cynomolgus monkeys
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-020-16173-0
– volume: 24
  start-page: 268
  year: 2022
  end-page: 278
  ident: CR59
  article-title: dCas9-based gene editing for cleavage-free genomic knock-in of long sequences
  publication-title: Nat. Cell Biol.
  doi: 10.1038/s41556-021-00836-1
– volume: 38
  start-page: 892
  year: 2020
  end-page: 900
  ident: CR13
  article-title: Directed evolution of adenine base editors with increased activity and therapeutic application
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0491-6
– volume: 36
  start-page: 324
  year: 2018
  end-page: 327
  ident: CR28
  article-title: Base editing with a Cpf1–cytidine deaminase fusion
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.4102
– volume: 165
  start-page: 949
  year: 2016
  end-page: 962
  ident: CR22
  article-title: Crystal structure of Cpf1 in complex with guide RNA and target DNA
  publication-title: Cell
  doi: 10.1016/j.cell.2016.04.003
– volume: 44
  start-page: W54
  year: 2016
  end-page: W57
  ident: CR32
  article-title: tRNAscan-SE On-line: integrating search and context for analysis of transfer RNA genes
  publication-title: Nucleic acids Res.
  doi: 10.1093/nar/gkw413
– volume: 602
  start-page: 455
  year: 2022
  end-page: 460
  ident: CR65
  article-title: Genome-edited powdery mildew resistance in wheat without growth penalties
  publication-title: Nature
  doi: 10.1038/s41586-022-04395-9
– volume: 10
  year: 2019
  ident: CR12
  article-title: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-019-13007-6
– volume: 29
  start-page: 3107
  year: 2021
  end-page: 3124
  ident: CR7
  article-title: In vivo somatic cell base editing and prime editing
  publication-title: Mol. Ther.
  doi: 10.1016/j.ymthe.2021.09.002
– volume: 3
  start-page: 17018
  year: 2017
  ident: CR27
  article-title: A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants
  publication-title: Nat. Plants
  doi: 10.1038/nplants.2017.18
– volume: 185
  start-page: 250
  year: 2022
  end-page: 265.e216
  ident: CR64
  article-title: Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins
  publication-title: Cell
  doi: 10.1016/j.cell.2021.12.021
– volume: 31
  start-page: 827
  year: 2013
  end-page: 832
  ident: CR52
  article-title: DNA targeting specificity of RNA-guided Cas9 nucleases
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.2647
– volume: 532
  start-page: 517
  year: 2016
  end-page: 521
  ident: CR15
  article-title: The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
  publication-title: Nature
  doi: 10.1038/nature17945
– volume: 571
  start-page: 275
  year: 2019
  end-page: 278
  ident: CR38
  article-title: Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis
  publication-title: Nature
  doi: 10.1038/s41586-019-1314-0
– volume: 13
  start-page: 313
  year: 2018
  end-page: 325
  ident: CR56
  article-title: The future of multiplexed eukaryotic genome engineering
  publication-title: ACS Chem. Biol.
  doi: 10.1021/acschembio.7b00842
– volume: 533
  start-page: 420
  year: 2016
  end-page: 424
  ident: CR1
  article-title: Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
  publication-title: Nature
  doi: 10.1038/nature17946
– volume: 25
  start-page: 776
  year: 2019
  end-page: 783
  ident: CR45
  article-title: Highly efficient therapeutic gene editing of human hematopoietic stem cells
  publication-title: Nat. Med.
  doi: 10.1038/s41591-019-0401-y
– volume: 7
  start-page: eabg4910
  year: 2021
  ident: CR9
  article-title: Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing
  publication-title: Sci. Adv.
  doi: 10.1126/sciadv.abg4910
– volume: 38
  start-page: 471
  year: 2020
  end-page: 481
  ident: CR25
  article-title: Continuous evolution of SpCas9 variants compatible with non-G PAMs
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0412-8
– ident: CR62
– volume: 339
  start-page: 819
  year: 2013
  end-page: 823
  ident: CR10
  article-title: Multiplex genome engineering using CRISPR/Cas systems
  publication-title: Science
  doi: 10.1126/science.1231143
– ident: CR24
– volume: 8
  start-page: 1
  year: 2018
  end-page: 14
  ident: CR34
  article-title: A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-018-31476-5
– volume: 21
  start-page: 1683
  year: 2015
  end-page: 1689
  ident: CR39
  article-title: Expression of CRISPR/Cas single guide RNAs using small tRNA promoters
  publication-title: RNA
  doi: 10.1261/rna.051631.115
– volume: 13
  start-page: 358
  year: 2018
  end-page: 376
  ident: CR49
  article-title: CRISPR/Cas9 genome editing in human hematopoietic stem cells
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2017.143
– ident: 30514_CR57
– volume: 39
  start-page: 94
  year: 2021
  ident: 30514_CR20
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0600-6
– volume: 16
  start-page: 887
  year: 2019
  ident: 30514_CR19
  publication-title: Nat. Methods
  doi: 10.1038/s41592-019-0508-6
– volume: 571
  start-page: 275
  year: 2019
  ident: 30514_CR38
  publication-title: Nature
  doi: 10.1038/s41586-019-1314-0
– volume: 44
  start-page: W54
  year: 2016
  ident: 30514_CR32
  publication-title: Nucleic acids Res.
  doi: 10.1093/nar/gkw413
– ident: 30514_CR24
  doi: 10.1126/sciadv.aau0766
– volume: 11
  year: 2020
  ident: 30514_CR11
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-020-15053-x
– volume: 373
  start-page: 882
  year: 2021
  ident: 30514_CR63
  publication-title: Science
  doi: 10.1126/science.abg6155
– volume: 24
  start-page: 268
  year: 2022
  ident: 30514_CR59
  publication-title: Nat. Cell Biol.
  doi: 10.1038/s41556-021-00836-1
– volume: 339
  start-page: 819
  year: 2013
  ident: 30514_CR10
  publication-title: Science
  doi: 10.1126/science.1231143
– volume: 12
  start-page: 1
  year: 2021
  ident: 30514_CR67
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-021-21337-7
– ident: 30514_CR68
  doi: 10.1038/s41467-022-30514-1
– volume: 589
  start-page: 608
  year: 2021
  ident: 30514_CR8
  publication-title: Nature
  doi: 10.1038/s41586-020-03086-7
– volume: 551
  start-page: 464
  year: 2017
  ident: 30514_CR2
  publication-title: Nature
  doi: 10.1038/nature24644
– volume: 10
  year: 2019
  ident: 30514_CR12
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-019-13007-6
– volume: 368
  start-page: 290
  year: 2020
  ident: 30514_CR26
  publication-title: Science
  doi: 10.1126/science.aba8853
– volume: 533
  start-page: 420
  year: 2016
  ident: 30514_CR1
  publication-title: Nature
  doi: 10.1038/nature17946
– ident: 30514_CR61
  doi: 10.1038/s41586-022-04494-7
– volume: 112
  start-page: 3570
  year: 2015
  ident: 30514_CR31
  publication-title: Proc. Natl Acad. Sci.
  doi: 10.1073/pnas.1420294112
– volume: 165
  start-page: 949
  year: 2016
  ident: 30514_CR22
  publication-title: Cell
  doi: 10.1016/j.cell.2016.04.003
– volume: 3
  start-page: 17018
  year: 2017
  ident: 30514_CR27
  publication-title: Nat. Plants
  doi: 10.1038/nplants.2017.18
– volume: 21
  start-page: 1683
  year: 2015
  ident: 30514_CR39
  publication-title: RNA
  doi: 10.1261/rna.051631.115
– volume: 33
  start-page: 187
  year: 2015
  ident: 30514_CR44
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.3117
– volume: 1
  start-page: 239
  year: 2018
  ident: 30514_CR40
  publication-title: CRISPR J.
  doi: 10.1089/crispr.2018.0014
– volume: 576
  start-page: 149
  year: 2019
  ident: 30514_CR3
  publication-title: Nature
  doi: 10.1038/s41586-019-1711-4
– volume: 44
  start-page: D184
  year: 2016
  ident: 30514_CR33
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkv1309
– volume: 532
  start-page: 517
  year: 2016
  ident: 30514_CR15
  publication-title: Nature
  doi: 10.1038/nature17945
– volume: 39
  start-page: 1414
  year: 2021
  ident: 30514_CR4
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-021-00938-z
– volume: 482
  start-page: 889
  year: 2017
  ident: 30514_CR53
  publication-title: Biochem. Biophys. Res. Commun.
  doi: 10.1016/j.bbrc.2016.11.129
– volume: 24
  start-page: 590
  year: 2022
  ident: 30514_CR21
  publication-title: Nat. Cell Biol.
  doi: 10.1038/s41556-022-00870-7
– volume: 361
  start-page: 1259
  year: 2018
  ident: 30514_CR23
  publication-title: Science
  doi: 10.1126/science.aas9129
– volume: 13
  start-page: 313
  year: 2018
  ident: 30514_CR56
  publication-title: ACS Chem. Biol.
  doi: 10.1021/acschembio.7b00842
– volume: 37
  start-page: 224
  year: 2019
  ident: 30514_CR41
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-019-0032-3
– volume: 39
  start-page: 41
  year: 2021
  ident: 30514_CR43
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0609-x
– volume: 54
  start-page: 698
  year: 2014
  ident: 30514_CR55
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2014.04.022
– volume: 25
  start-page: 776
  year: 2019
  ident: 30514_CR45
  publication-title: Nat. Med.
  doi: 10.1038/s41591-019-0401-y
– volume: 569
  start-page: 433
  year: 2019
  ident: 30514_CR36
  publication-title: Nature
  doi: 10.1038/s41586-019-1161-z
– ident: 30514_CR62
  doi: 10.1126/science.abj4008
– volume: 36
  start-page: 843
  year: 2018
  ident: 30514_CR35
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.4172
– volume: 38
  start-page: 824
  year: 2020
  ident: 30514_CR6
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0561-9
– volume: 7
  start-page: eabg4910
  year: 2021
  ident: 30514_CR9
  publication-title: Sci. Adv.
  doi: 10.1126/sciadv.abg4910
– volume: 35
  start-page: 31
  year: 2017
  ident: 30514_CR16
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.3737
– volume: 38
  start-page: 620
  year: 2020
  ident: 30514_CR42
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0414-6
– volume: 11
  year: 2020
  ident: 30514_CR14
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-020-16173-0
– volume: 37
  start-page: 276
  year: 2019
  ident: 30514_CR29
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-018-0011-0
– volume: 8
  start-page: 1
  year: 2018
  ident: 30514_CR34
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-018-31476-5
– volume: 287
  start-page: 28686
  year: 2012
  ident: 30514_CR47
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M112.372680
– volume: 602
  start-page: 455
  year: 2022
  ident: 30514_CR65
  publication-title: Nature
  doi: 10.1038/s41586-022-04395-9
– volume: 29
  start-page: 3107
  year: 2021
  ident: 30514_CR7
  publication-title: Mol. Ther.
  doi: 10.1016/j.ymthe.2021.09.002
– volume: 13
  start-page: 839
  year: 2017
  ident: 30514_CR17
  publication-title: Nat. Chem. Biol.
  doi: 10.1038/nchembio.2410
– volume: 26
  start-page: 535
  year: 2020
  ident: 30514_CR46
  publication-title: Nat. Med.
  doi: 10.1038/s41591-020-0790-y
– ident: 30514_CR58
  doi: 10.1101/2021.11.01.466786
– volume: 36
  start-page: 324
  year: 2018
  ident: 30514_CR28
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.4102
– volume: 11
  start-page: 783
  year: 2014
  ident: 30514_CR51
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.3047
– volume: 16
  start-page: 1089
  year: 2021
  ident: 30514_CR5
  publication-title: Nat. Protoc.
  doi: 10.1038/s41596-020-00450-9
– volume: 38
  start-page: 892
  year: 2020
  ident: 30514_CR13
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0491-6
– volume: 13
  start-page: e0198714
  year: 2018
  ident: 30514_CR54
  publication-title: PLOS ONE
  doi: 10.1371/journal.pone.0198714
– volume: 8
  start-page: 566
  year: 2003
  ident: 30514_CR66
  publication-title: J. Biomol. Screen.
  doi: 10.1177/1087057103256465
– volume: 4
  start-page: 97
  year: 2020
  ident: 30514_CR48
  publication-title: Nat. Biomed. Eng.
  doi: 10.1038/s41551-019-0501-5
– volume: 31
  start-page: 827
  year: 2013
  ident: 30514_CR52
  publication-title: Nat. Biotechnol.
  doi: 10.1038/nbt.2647
– volume: 13
  start-page: 358
  year: 2018
  ident: 30514_CR49
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2017.143
– volume: 185
  start-page: 250
  year: 2022
  ident: 30514_CR64
  publication-title: Cell
  doi: 10.1016/j.cell.2021.12.021
– volume: 24
  start-page: 573
  year: 1981
  ident: 30514_CR37
  publication-title: Cell
  doi: 10.1016/0092-8674(81)90348-2
– volume: 38
  start-page: 883
  year: 2020
  ident: 30514_CR30
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0453-z
– volume: 370
  start-page: eaaz6063
  year: 2020
  ident: 30514_CR60
  publication-title: Science
  doi: 10.1126/science.aaz6063
– volume: 14
  start-page: 1163
  year: 2017
  ident: 30514_CR18
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.4483
– volume: 10
  year: 2013
  ident: 30514_CR50
  publication-title: Virol. J.
  doi: 10.1186/1743-422X-10-74
– volume: 38
  start-page: 471
  year: 2020
  ident: 30514_CR25
  publication-title: Nat. Biotechnol.
  doi: 10.1038/s41587-020-0412-8
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Snippet Current base- and prime-editing technologies lack efficient strategies to edit multiple genomic loci simultaneously, limiting their applications in complex...
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SubjectTerms 45/22
45/23
45/29
45/91
631/208
631/337/4041/3196
631/61
Arrays
Clustered Regularly Interspaced Short Palindromic Repeats - genetics
Constraining
CRISPR
CRISPR-Cas Systems - genetics
DNA-directed RNA polymerase
Editing
Gene Editing
Gene expression
Gene loci
Genomics
gRNA
Humanities and Social Sciences
Humans
Medical research
multidisciplinary
Multiplexing
RNA polymerase
RNA, Guide, CRISPR-Cas Systems - genetics
RNA, Transfer - genetics
Science
Science (multidisciplinary)
Therapeutic applications
Transfer RNA
tRNA Cys
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Title Multiplex base- and prime-editing with drive-and-process CRISPR arrays
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Volume 13
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